Validation of the RIDASCREEN® Peanut for Determination of Peanut Protein in Cookies, Milk Chocolate, Ice Cream, Trail Mix, Puffed Rice Cereals, and Granola Bar: AOAC Performance Tested MethodSM 112102
| العنوان: | Validation of the RIDASCREEN® Peanut for Determination of Peanut Protein in Cookies, Milk Chocolate, Ice Cream, Trail Mix, Puffed Rice Cereals, and Granola Bar: AOAC Performance Tested MethodSM 112102 |
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| المؤلفون: | Markus Lacorn, Tina Dubois, Christian Gößwein, Rebecca Kredel, Bianca Ferkinghoff, Sharon Brunelle, Jérémie Théolier, Silvia Dominguez, Thomas Weiss |
| المصدر: | Journal of AOAC INTERNATIONAL. 105:784-801 |
| بيانات النشر: | Oxford University Press (OUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Pharmacology, Arachis, Ice Cream, Enzyme-Linked Immunosorbent Assay, Oryza, Allergens, Analytical Chemistry, Animals, Humans, Environmental Chemistry, Chocolate, Edible Grain, Agronomy and Crop Science, Food Analysis, Food Science |
| الوصف: | Background Regulations in many countries worldwide prescribe that peanut must be listed on food labels as a cause of food allergies. Re-evaluated voluntary incidental trace allergen labelling (VITAL) values for peanut revealed the eliciting dose (ED01) value—at which 99% of all peanut-allergic individuals will not react—is 0.2 mg peanut protein. Objective Validation of a sandwich ELISA based on monoclonal antibodies to detect peanut proteins. Methods Non-processed and processed samples are extracted by an easy procedure at 60°C within 10 min. The measurement range is between 0.75 and 6 mg/kg peanut using a national institute of standards and technology (NIST) reference material as calibrator. Results The system shows no cross-reactivity against 91 different food commodities. The LOD was 0.15 mg/kg for food matrixes such as cookies, milk chocolate, ice cream, trail mix, puffed rice cereal, and granola bar. LOQ was verified at a level of 0.75 mg/kg. Recovery studies with incurred milk chocolate and ice cream revealed consistent recoveries between 67 and 85%. Mean recoveries for incurred cookies depend on the baking temperature and time and ranged from 60 to 109%. Repeatability was between 5.2 and 12.3%, whereas relative intermediate precision was between 6.4 and 13.0%. The results for incurred cookies and milk chocolate in the independent laboratory study showed mean recoveries between 99 and 104% with RSDs between 3.56 and 19.5% under repeatability conditions. Conclusion The results from the in-house validation study and the independent lab confirmed that the method is accurate and in accordance with requirements laid down in Standard Method Performance Requirement 2017.020. Highlights RIDASCREEN® Peanut quantifies proteins from peanut in a wide range of food categories. |
| تدمد: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoacint/qsab168 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c74fcf4989276cdec75523819de1bc2 https://doi.org/10.1093/jaoacint/qsab168 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9c74fcf4989276cdec75523819de1bc2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19447922 10603271 |
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| DOI: | 10.1093/jaoacint/qsab168 |