Stabilization of β-catenin promotes melanocyte specification at the expense of the Schwann cell lineage
| العنوان: | Stabilization of β-catenin promotes melanocyte specification at the expense of the Schwann cell lineage |
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| المؤلفون: | Sophie Colombo, Valérie Petit, Roselyne Y. Wagner, Delphine Champeval, Ichiro Yajima, Franck Gesbert, Zackie Aktary, Irwin Davidson, Véronique Delmas, Lionel Larue |
| المساهمون: | delmas, veronique, Signalisation, radiobiologie et cancer, Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Development (Cambridge, England) Development (Cambridge, England), 2022, 149 (2), pp.dev194407. ⟨10.1242/dev.194407⟩ |
| بيانات النشر: | HAL CCSD, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Mitf, Mouse, Proliferation, FoxD3, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Mice, Cell Line, Tumor, [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Animals, Humans, Cell Lineage, Molecular Biology, Wnt Signaling Pathway, Determination, [SDV.BDD]Life Sciences [q-bio]/Development Biology, beta Catenin, Microphthalmia-Associated Transcription Factor, Protein Stability, Pigmentation, Cell Differentiation, Forkhead Transcription Factors, Mice, Inbred C57BL, Repressor Proteins, Cell fate, Melanocytes, Schwann Cells, Developmental Biology |
| الوصف: | The canonical Wnt/β-catenin pathway governs a multitude of developmental processes in various cell lineages, including the melanocyte lineage. Indeed, β-catenin regulates transcription of Mitf-M, the master regulator of this lineage. The first wave of melanocytes to colonize the skin is directly derived from neural crest cells, whereas the second wave of melanocytes is derived from Schwann cell precursors (SCPs). We investigated the influence of β-catenin in the development of melanocytes of the first and second waves by generating mice expressing a constitutively active form of β-catenin in cells expressing tyrosinase. Constitutive activation of β-catenin did not affect the development of truncal melanoblasts but led to marked hyperpigmentation of the paws. By activating β-catenin at various stages of development (E8.5-E11.5), we showed that the activation of β-catenin in bipotent SCPs favored melanoblast specification at the expense of Schwann cells in the limbs within a specific temporal window. Furthermore, in vitro hyperactivation of the Wnt/β-catenin pathway, which is required for melanocyte development, induces activation of Mitf-M, in turn repressing FoxD3 expression. In conclusion, β-catenin overexpression promotes SCP cell fate decisions towards the melanocyte lineage. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0950-1991 1477-9129 |
| DOI: | 10.1242/dev.194407⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b154cee5075105d5239a630c4e512b5 https://hal.science/hal-03780846 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9b154cee5075105d5239a630c4e512b5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09501991 14779129 |
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| DOI: | 10.1242/dev.194407⟩ |