Sensitization of primary cultures from rat dorsal root ganglia with lipopolysaccharide (LPS) requires a robust inflammatory response
| العنوان: | Sensitization of primary cultures from rat dorsal root ganglia with lipopolysaccharide (LPS) requires a robust inflammatory response |
|---|---|
| المؤلفون: | Franz Nürnberger, Stephan Leisengang, Daniela Ott, Jolanta Murgott, Rüdiger Gerstberger, Christoph Rummel, Joachim Roth |
| المصدر: | Inflammation Research |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | LPS sensitization, Inflammation, Lipopolysaccharides, Pharmacology, Dose-Response Relationship, Drug, Tumor Necrosis Factor-alpha, Short Communication, Immunology, Rats, Ca2+-imaging, Ganglia, Spinal, Mixed neuro-glial cultures, Cytokines, Animals, lipids (amino acids, peptides, and proteins), Calcium Signaling, Capsaicin, Rats, Wistar, Dorsal root ganglia, Cells, Cultured |
| الوصف: | Objective We investigated whether it is possible to induce a state of “LPS-sensitization” in neurons of primary cultures from rat dorsal root ganglia by pre-treatment with ultra-low doses of LPS. Methods DRG primary cultures were pre-treated with low to ultra-low doses of LPS (0.001–0.1 µg/ml) for 18 h, followed by a short-term stimulation with a higher LPS-dose (10 µg/ml for 2 h). TNF-α in the supernatants was measured as a sensitive read out. Using the fura-2 340/380 nm ratio imaging technique, we further investigated the capsaicin-evoked Ca2+-signals in neurons from DRG, which were pre-treated with a wide range of LPS-doses. Results Release of TNF-α evoked by stimulation with 10 µg/ml LPS into the supernatant was not significantly modified by pre-exposure to low to ultra-low LPS-doses. Capsaicin-evoked Ca2+-signals were significantly enhanced by pre-treatment with LPS doses being above a certain threshold. Conclusion Ultra-low doses of LPS, which per se do not evoke a detectable inflammatory response, are not sufficient to sensitize neurons (Ca2+-responses) and glial elements (TNF-α-responses) of the primary afferent somatosensory system. |
| تدمد: | 1420-908X 1023-3830 |
| DOI: | 10.1007/s00011-021-01534-2 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9aaaf64c1b64cba83d1a3ae13d6e8194 https://doi.org/10.1007/s00011-021-01534-2 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9aaaf64c1b64cba83d1a3ae13d6e8194 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1420908X 10233830 |
|---|---|
| DOI: | 10.1007/s00011-021-01534-2 |