Increased Expression of N2BA Titin Corresponds to More Compliant Myofibrils in Athlete's Heart
| العنوان: | Increased Expression of N2BA Titin Corresponds to More Compliant Myofibrils in Athlete's Heart |
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| المؤلفون: | Tamás Radovits, Dalma Kellermayer, Attila Oláh, Bálint Kiss, Miklós S.Z. Kellermayer, Béla Merkely, Henk Granzier, Hedvig Tordai |
| المصدر: | International Journal of Molecular Sciences Volume 22 Issue 20 International Journal of Molecular Sciences, Vol 22, Iss 11110, p 11110 (2021) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, Sarcomere, Myofibrils, transverse stiffness, Protein Isoforms, Connectin, Cardiomegaly, Exercise-Induced, Biology (General), Spectroscopy, biology, Cardiac muscle, Heart, General Medicine, musculoskeletal system, Adaptation, Physiological, Computer Science Applications, Cardiac myofibril, Chemistry, medicine.anatomical_structure, Cardiology, cardiovascular system, cardiac myofibril, Titin, AFM, Gene isoform, Sarcomeres, medicine.medical_specialty, animal structures, QH301-705.5, Athlete's heart, macromolecular substances, Catalysis, Article, Inorganic Chemistry, Internal medicine, Elastic Modulus, Physical Conditioning, Animal, medicine, Animals, titin, Physical and Theoretical Chemistry, Rats, Wistar, QD1-999, Molecular Biology, business.industry, Myocardium, Organic Chemistry, Myocardial Contraction, Rats, Disease Models, Animal, Ventricle, biology.protein, Myofibril, business, athlete’s heart |
| الوصف: | Long-term exercise induces physiological cardiac adaptation, a condition referred to as athlete’s heart. Exercise tolerance is known to be associated with decreased cardiac passive stiffness. Passive stiffness of the heart muscle is determined by the giant elastic protein titin. The adult cardiac muscle contains two titin isoforms: the more compliant N2BA and the stiffer N2B. Titin-based passive stiffness may be controlled by altering the expression of the different isoforms or via post-translational modifications such as phosphorylation. Currently, there is very limited knowledge about titin’s role in cardiac adaptation during long-term exercise. Our aim was to determine the N2BA/N2B ratio and post-translational phosphorylation of titin in the left ventricle and to correlate the changes with the structure and transverse stiffness of cardiac sarcomeres in a rat model of an athlete’s heart. The athlete’s heart was induced by a 12-week-long swim-based training. In the exercised myocardium the N2BA/N2B ratio was significantly increased, Ser11878 of the PEVK domain was hypophosphorlyated, and the sarcomeric transverse elastic modulus was reduced. Thus, the reduced passive stiffness in the athlete’s heart is likely caused by a shift towards the expression of the longer cardiac titin isoform and a phosphorylation-induced softening of the PEVK domain which is manifested in a mechanical rearrangement locally, within the cardiac sarcomere. |
| وصف الملف: | application/pdf |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a8a5d05401789cbf87973c45eb48d44 https://pubmed.ncbi.nlm.nih.gov/34681770 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9a8a5d05401789cbf87973c45eb48d44 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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