Cholesterol Efflux Efficiency of Reconstituted HDL Is Affected by Nanoparticle Lipid Composition
| العنوان: | Cholesterol Efflux Efficiency of Reconstituted HDL Is Affected by Nanoparticle Lipid Composition |
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| المؤلفون: | Iraide Alloza, Unai Galicia-Garcia, Asier Benito-Vicente, Asier Larrea-Sebal, César Martín, Koen Vandenbroeck, Helena Ostolaza, Shifa Jebari-Benslaiman, Kepa B. Uribe |
| المصدر: | Addi: Archivo Digital para la Docencia y la Investigación Universidad del País Vasco Biomedicines Volume 8 Issue 10 Addi. Archivo Digital para la Docencia y la Investigación instname Biomedicines, Vol 8, Iss 373, p 373 (2020) |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Medicine (miscellaneous), 030204 cardiovascular system & hematology, Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, In vivo, cardiovascular disease, lcsh:QH301-705.5, apoA-I, Nanodisc, rHDL, biology, Cholesterol, Bilayer, technology, industry, and agriculture, Transporter, 030104 developmental biology, lcsh:Biology (General), chemistry, ABCA1, Cholesteryl ester, biology.protein, Biophysics, lipids (amino acids, peptides, and proteins), Efflux, nanodisc, cholesterol efflux |
| الوصف: | Cardiovascular disease (CVD), the leading cause of mortality worldwide is primarily caused by atherosclerosis, which is promoted by the accumulation of low-density lipoproteins into the intima of large arteries. Multiple nanoparticles mimicking natural HDL (rHDL) have been designed to remove cholesterol excess in CVD therapy. The goal of this investigation was to assess the cholesterol efflux efficiency of rHDLs with different lipid compositions, mimicking different maturation stages of high-density lipoproteins (HDLs) occurring in vivo. Methods: the cholesterol efflux activity of soybean PC (Soy-PC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), DPPC:Chol:1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine (LysoPC) and DPPC:18:2 cholesteryl ester (CE):LysoPC rHDLs was determined in several cell models to investigate the contribution of lipid composition to the effectiveness of cholesterol removal. Results: DPPC rHDLs are the most efficient particles, inducing cholesterol efflux in all cellular models and in all conditions the effect was potentiated when the ABCA1 transporter was upregulated. Conclusions: DPPC rHDLs, which resemble nascent HDL, are the most effective particles in inducing cholesterol efflux due to the higher physical binding affinity of cholesterol to the saturated long-chain-length phospholipids and the favored cholesterol transfer from a highly positively curved bilayer, to an accepting planar bilayer such as DPPC rHDLs. The physicochemical characteristics of rHDLs should be taken into consideration to design more efficient nanoparticles to promote cholesterol efflux. This work was supported by the Basque Government (Grupos Consolidados IT-1264-19). U.G.-G. was supported by Fundación Biofísica Bizkaia. A.B.-V. was supported by Programa de especialización de Personal Investigador Doctor en la UPV/EHU (2019) 2019–2020. S.J.-B. and A.L.-S. were supported by a grant PIF (2017–2018) and (2019–2020), Gobierno Vasco, respectively. A.L.-S. was partially supported by Fundación Biofísica Bizkaia. |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a32c088263e321f44bc679bd52384a3 http://hdl.handle.net/10810/47301 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9a32c088263e321f44bc679bd52384a3 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |