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Fructose overfeeding in first-degree relatives of type 2 diabetic patients impacts energy metabolism and mitochondrial functions in skeletal muscle

التفاصيل البيبلوغرافية
العنوان: Fructose overfeeding in first-degree relatives of type 2 diabetic patients impacts energy metabolism and mitochondrial functions in skeletal muscle
المؤلفون: Emmanuel Disse, Serge Nataf, Luc Tappy, Laurent Pays, Emmanuelle Meugnier, Martine Laville, Hubert Vidal, Kim-Anne Lê, J. Brozek, Emilie Blond, Kevin Seyssel, Christine Durand
المساهمون: Department of Physiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Physiologie, Université de Lausanne (UNIL), Centre Camille Jullian - Histoire et archéologie de la Méditerranée et de l'Afrique du Nord de la protohistoire à la fin de l'Antiquité (CCJ), Aix Marseille Université (AMU)-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS), Genfit, Entreprise biopharmaceutique GENFIT Loos, Centre de Recherche en Nutrition Humaine Rhône-Alpes (CRNH-RH), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-CHU Saint-Etienne-Hospices Civils de Lyon (HCL)-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), CRNH, Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Faculté Biologie et Médecine, Département Physiologie, Université de Lausanne, Banque Cellules et Tissus, Hôpital Edouard Herriot [CHU - HCL], Swiss National Science Foundation 310000-109737/1, Hospices Civils de Lyon, ISI program of Agence pour l'Innovation OSEO (IT-DIAB project), Ministere de l'Enseignement Superieur et de la Recherche, France, Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA)
المصدر: Molecular Nutrition & Food Research
Molecular Nutrition & Food Research, 2016, 60 (12), pp.2691-2699
Molecular Nutrition and Food Research
Molecular Nutrition and Food Research, Wiley-VCH Verlag, 2016, 60 (12), pp.2691-2699. ⟨10.1002/mnfr.201600407⟩
سنة النشر: 2016
مصطلحات موضوعية: 0301 basic medicine, Male, [SDV]Life Sciences [q-bio], Muscle Fibers, Skeletal, Type 2 diabetes, chemistry.chemical_compound, 0302 clinical medicine, lipid oxidation, Nuclear Receptor Subfamily 4, Group A, Member 2, Beta oxidation, ComputingMilieux_MISCELLANEOUS, gène régulateur, 2. Zero hunger, Cross-Over Studies, Myogenesis, muscle squelettique, Mitochondria, medicine.anatomical_structure, Biotechnology, Adult, medicine.medical_specialty, regulatory gene, diabète de type 2, Offspring, métabolisme énergétique, 030209 endocrinology & metabolism, oxydation lipidique, Fructose, Biology, biopsie, Cell Line, 03 medical and health sciences, Young Adult, Lipid oxidation, oxydation des lipides, Internal medicine, medicine, Humans, biopsy, PPAR alpha, skeletal muscle, Gene Expression Profiling, Skeletal muscle, medicine.disease, Lipid Metabolism, type ii diabetes, étude transcriptomique, Diet, 030104 developmental biology, Endocrinology, voluntary muscle, chemistry, Diabetes Mellitus, Type 2, Uric acid, gene regulation, Energy Metabolism, Transcriptome, Food Science, high-fructose diet
الوصف: Scope: The aim of the study was to assess the effects of a high-fructose diet (HFrD) on skeletal muscle transcriptomic response in healthy offspring of patients with type 2 diabetes, a subgroup of individuals prone to metabolic disorders.Methods and results: Ten healthy normal weight first-degree relatives of type 2 diabetic patients were submitted to a HFrD (+ 3.5 g fructose/kg fat-free mass per day) during 7 days. A global transcriptomic analysis was performed on skeletal muscle biopsies combined with in vitro experiments using primary myotubes. Transcriptomic analysis highlighted profound effects on fatty acid oxidation and mitochondrial pathways supporting the whole-body metabolic shift with the preferential use of carbohydrates instead of lipids. Bioinformatics tools pointed out possible transcription factors orchestrating this genomic regulation, such as PPAR alpha and NR4A2. In vitro experiments in human myotubes suggested an indirect action of fructose in skeletal muscle, which seemed to be independent from lactate, uric acid, or nitric oxide.Conclusion: This study shows therefore that a large cluster of genes related to energy metabolism, mitochondrial function, and lipid oxidation was downregulated after 7 days of HFrD, thus supporting the concept that overconsumption of fructose-containing foods could contribute to metabolic deterioration in humans.
تدمد: 1613-4133
1613-4125
DOI: 10.1002/mnfr.201600407⟩
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99d31daee63b3610dd9f18d95803dd3b
https://pubmed.ncbi.nlm.nih.gov/27468128
Rights: CLOSED
رقم الانضمام: edsair.doi.dedup.....99d31daee63b3610dd9f18d95803dd3b
قاعدة البيانات: OpenAIRE
الوصف
تدمد:16134133
16134125
DOI:10.1002/mnfr.201600407⟩