Role of von Willebrand factor associated to extracellular matrices in platelet adhesion
| العنوان: | Role of von Willebrand factor associated to extracellular matrices in platelet adhesion |
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| المؤلفون: | Corinne Marteaux, Cécile V. Denis, Laure Coulombel, Dominique Baruch, Dominique Meyer, Damien Schoevaert |
| المصدر: | Blood. 77:519-527 |
| بيانات النشر: | American Society of Hematology, 1991. |
| سنة النشر: | 1991 |
| مصطلحات موضوعية: | congenital, hereditary, and neonatal diseases and abnormalities, medicine.drug_class, Platelet adhesion, Immunology, Dose dependence, Monoclonal antibody, Biochemistry, Extracellular matrix, Platelet Adhesiveness, Von Willebrand factor, hemic and lymphatic diseases, von Willebrand Factor, medicine, Extracellular, Humans, Platelet, Cells, Cultured, biology, Chemistry, Cell Biology, Hematology, Adhesion, Fibroblasts, Extracellular Matrix, Cell biology, cardiovascular system, biology.protein, Endothelium, Vascular, circulatory and respiratory physiology |
| الوصف: | The respective role of plasmatic and endothelial extracellular matrix (ECM)-associated von Willebrand factor (vWF) in platelet adhesion was investigated at a high shear rate using a parallel-plate perfusion chamber. Incubation of the endothelial ECM with a monoclonal antibody (MoAb) to vWF, which specifically blocks vWF binding to platelet GP Ib (MoAb 322), inhibited 45% of platelet adhesion. Complete inhibition was achieved by incubating both plasma and endothelial ECM with MoAb 322 at concentrations that blocked only about 50% of adhesion when added separately. The effect of ECM-associated vWF was further demonstrated when a fibroblastic ECM, normally devoid of vWF, was coated with purified plasmatic vWF. Matrix associated-vWF was able to significantly enhance platelet adhesion in both the presence and the absence of plasmatic vWF. In contrast, this effect was not seen on endothelial ECM. Binding of exogenous vWF to the ECM was specific and dose dependent, reached the same value (500 ng/cm2) on both fibroblastic ECM and endothelial ECM, but exhibited a threefold-lower apparent dissociation constant (KD) on fibroblastic than on endothelial ECM. Our studies suggest that vWF deposited by endothelial cells in the ECM may be the most active form in platelet adhesion, whereas plasmatic vWF may only play a secondary role. |
| تدمد: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v77.3.519.519 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::998f469d0e4bd65aff5ed4b17911f1c3 https://doi.org/10.1182/blood.v77.3.519.519 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....998f469d0e4bd65aff5ed4b17911f1c3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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| DOI: | 10.1182/blood.v77.3.519.519 |