Radiation damage to biological samples: still a pertinent issue
| العنوان: | Radiation damage to biological samples: still a pertinent issue |
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| المؤلفون: | Martin Weik, Elspeth F. Garman |
| المصدر: | Journal of Synchrotron Radiation |
| بيانات النشر: | International Union of Crystallography (IUCr), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Nuclear and High Energy Physics, single-wavelength anomalous dispersion, Materials science, X-ray footprinting, Synchrotron radiation, Electron, law.invention, macromolecular crystallography, law, Radiation damage, serial crystallography, Instrumentation, Radiation, electron microscopy, Scattering, X-ray imaging, dose, SAXS, Synchrotron, Computational physics, Beamline, radiation damage, XFEL simulations, Absorbed dose, Radiolysis, room-temperature crystallography |
| الوصف: | An understanding of radiation damage effects suffered by biological samples during structural analysis using both X-rays and electrons is pivotal to obtain reliable molecular models of imaged molecules. This special issue on radiation damage contains six papers reporting analyses of damage from a range of biophysical imaging techniques. An understanding of radiation damage effects suffered by biological samples during structural analysis using both X-rays and electrons is pivotal to obtain reliable molecular models of imaged molecules. This special issue on radiation damage contains six papers reporting analyses of damage from a range of biophysical imaging techniques. For X-ray diffraction, an in-depth study of multi-crystal small-wedge data collection single-wavelength anomalous dispersion phasing protocols is presented, concluding that an absorbed dose of 5 MGy per crystal was optimal to allow reliable phasing. For small-angle X-ray scattering, experiments are reported that evaluate the efficacy of three radical scavengers using a protein designed to give a clear signature of damage in the form of a large conformational change upon the breakage of a disulfide bond. The use of X-rays to induce OH radicals from the radiolysis of water for X-ray footprinting are covered in two papers. In the first, new developments and the data collection pipeline at the NSLS-II high-throughput dedicated synchrotron beamline are described, and, in the second, the X-ray induced changes in three different proteins under aerobic and low-oxygen conditions are investigated and correlated with the absorbed dose. Studies in XFEL science are represented by a report on simulations of ultrafast dynamics in protic ionic liquids, and, lastly, a broad coverage of possible methods for dose efficiency improvement in modalities using electrons is presented. These papers, as well as a brief synopsis of some other relevant literature published since the last Journal of Synchrotron Radiation Special Issue on Radiation Damage in 2019, are summarized below. |
| تدمد: | 1600-5775 |
| DOI: | 10.1107/s1600577521008845 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::976b07b751c7f1c0b9e982cb164fd3ce https://doi.org/10.1107/s1600577521008845 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....976b07b751c7f1c0b9e982cb164fd3ce |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16005775 |
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| DOI: | 10.1107/s1600577521008845 |