Isolation of RNA Polymerase from Clostridium difficile and Characterization of Glutamate Dehydrogenase and rRNA Gene Promoters In Vitro and In Vivo
| العنوان: | Isolation of RNA Polymerase from Clostridium difficile and Characterization of Glutamate Dehydrogenase and rRNA Gene Promoters In Vitro and In Vivo |
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| المؤلفون: | Abraham L. Sonenshein, Nagraj Mani, Bruno Dupuy |
| المساهمون: | Department of Molecular Biology and Microbiology [Tufts, Boston], Tufts University School of Medicine [Boston], Génétique Moléculaire Bactérienne, Institut Pasteur [Paris] (IP), This work was supported by research grants to A.L.S. (grants AI057637 and GM042219) and a project grant to the Tufts Gastroenterology Research and Secretory Processes Center (grant DK034928) from the U.S. Public Health Service, by a postdoctoral fellowship to N.M. from the Charles A. King Trust, and by research funds provided to B.D. by the Institut Pasteur, Paris, France., We thank L. Barosso and T. Wilkins for providing the cloned gdh promoter region, the Sanger Centre for making the C. difficile genome sequence available prior to publication, and B. Belitsky, K. Matsuno, and J. Singh for helpful discussions., Institut Pasteur [Paris] |
| المصدر: | Journal of Bacteriology Journal of Bacteriology, 2005, 188 (1), pp.96-102. ⟨10.1128/JB.188.1.96-102.2006⟩ Journal of Bacteriology, American Society for Microbiology, 2005, 188 (1), pp.96-102. ⟨10.1128/JB.188.1.96-102.2006⟩ |
| بيانات النشر: | American Society for Microbiology, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Transcription, Genetic, MESH: Clostridium difficile/genetics, [SDV]Life Sciences [q-bio], Molecular Sequence Data, MESH: Base Sequence, Biology, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Glutamate Dehydrogenase, MESH: Genes, rRNA, Transcription (biology), MESH: DNA-Directed RNA Polymerases/metabolism, RNA polymerase, Gene expression, Transcriptional regulation, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Clostridium difficile/enzymology, Promoter Regions, Genetic, Molecular Biology, Gene, MESH: Transcription, Genetic, 030304 developmental biology, Molecular Biology of Pathogens, Regulation of gene expression, MESH: Gene Expression Regulation, Bacterial, 0303 health sciences, MESH: Humans, MESH: Molecular Sequence Data, Base Sequence, Clostridioides difficile, 030306 microbiology, MESH: Glutamate Dehydrogenase/metabolism, Genes, rRNA, Promoter, DNA-Directed RNA Polymerases, Gene Expression Regulation, Bacterial, Clostridium difficile, Molecular biology, MESH: DNA-Directed RNA Polymerases/isolation & purification, MESH: Glutamate Dehydrogenase/genetics, 3. Good health, chemistry, MESH: DNA-Directed RNA Polymerases/genetics, MESH: Promoter Regions, Genetic*/physiology |
| الوصف: | Clostridium difficile is the primary causative agent of antibiotic-associated diarrheal disease. To facilitate molecular genetic analysis of gene expression in this organism, methods were developed to study transcriptional regulation in vitro and in vivo. That is, C. difficile RNA polymerase was partially purified and shown to bind to and initiate transcription in vitro from bona fide C. difficile promoters for rRNA and glutamate dehydrogenase genes. In addition, primer extension analyses and a β-glucuronidase reporter system were used to quantitate transcription from these promoters in vivo. With these tools in hand, it is now possible to characterize the behavior of any C. difficile gene in vivo and to study the regulation of its expression in detail. |
| تدمد: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.188.1.96-102.2006 |
| DOI: | 10.1128/JB.188.1.96-102.2006⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97250833acc57400f4b050a23355ace4 https://doi.org/10.1128/jb.188.1.96-102.2006 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....97250833acc57400f4b050a23355ace4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985530 00219193 |
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| DOI: | 10.1128/jb.188.1.96-102.2006 |