Canine Bone Marrow-derived Mesenchymal Stem Cells: Genomics, Proteomics and Functional Analyses of Paracrine Factors
| العنوان: | Canine Bone Marrow-derived Mesenchymal Stem Cells: Genomics, Proteomics and Functional Analyses of Paracrine Factors |
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| المؤلفون: | Michel Salzet, Adriana-Natalia Murgoci, Dasa Cizkova, Maria Kuricova, Štefan Čikoš, Martin Cente, Filip Humenik, Jana Farbakova, Soulaimane Aboulouard, Isabelle Fournier, Aladar Madari, Eva Petrovova, Zuzana Mojzisova, Alexandra Špirková, Dagmar Mudronova, Lenka Luptakova |
| المساهمون: | University of Veterinary Medicine and Pharmacy [Košice, Slovakia], Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 (PRISM), Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Slovak Academy of Sciences (SAS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), SALZET, Michel |
| المصدر: | Molecular and Cellular Proteomics Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2019, 18 (9), pp.1824-1835. ⟨10.1074/mcp.RA119.001507⟩ Molecular and Cellular Proteomics, 2019, 18 (9), pp.1824-1835. ⟨10.1074/mcp.RA119.001507⟩ Mol Cell Proteomics |
| بيانات النشر: | HAL CCSD, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, Proteomics, [SDV]Life Sciences [q-bio], Induced Pluripotent Stem Cells, Neovascularization, Physiologic, Bone Marrow Cells, Chick Embryo, Biology, Regenerative Medicine, Biochemistry, Regenerative medicine, Chorioallantoic Membrane, Analytical Chemistry, Canine, 03 medical and health sciences, Paracrine signalling, Dogs, Osteogenesis, Neurotrophic factors, Bone Marrow-Derived Stem Cells, Paracrine Communication, Animals, Cell Lineage, Progenitor cell, Induced pluripotent stem cell, Spinal Cord Injury, Molecular Biology, 030304 developmental biology, Secretome, 0303 health sciences, Adipogenesis, Research, Stem Cells, 030302 biochemistry & molecular biology, Mesenchymal stem cell, Proteins, Progenitor Cells, Proteomic, Cell Differentiation, Mesenchymal Stem Cells, 3. Good health, Cell biology, [SDV] Life Sciences [q-bio], Gene Expression Regulation, Culture Media, Conditioned, Biomarker: Prognostic, Stem cell, Biomarkers, Adult stem cell |
| الوصف: | International audience; Adult stem cells have become prominent candidates for treating various diseases in veterinary practice. The main goal of our study was therefore to provide a comprehensive study of canine bone marrow-derived mesenchymal stem cells (BMMSC) and conditioned media, isolated from healthy adult dogs of different breeds. Under well-defined standardized isolation protocols, the multipotent differentiation and specific surface markers of BMMSC were supplemented with their gene expression, proteomic profile, and their biological function. The presented data confirm that canine BMMSC express important genes for differentiation toward osteo-, chondro-, and tendo-genic directions, but also genes associated with angiogenic, neurotrophic, and immunomodulatory properties. Furthermore, using proteome profiling, we identify for the first time the dynamic release of various bioactive molecules, such as transcription and translation factors and osteogenic, growth, angiogenic, and neurotrophic factors from canine BMMSC conditioned medium. Importantly, the relevant genes were linked to their proteins as detected in the conditioned medium and further associated with angiogenic activity in chorioallantoic membrane (CAM) assay. In this way, we show that the canine BMMSC release a variety of bioactive molecules, revealing a strong paracrine component that may possess therapeutic potential in various pathologies. However, extensive experimental or preclinical trials testing canine sources need to be performed in order to better understand their paracrine action, which may lead to novel therapeutic strategies in veterinary medicine. |
| اللغة: | English |
| تدمد: | 1535-9476 1535-9484 |
| DOI: | 10.1074/mcp.RA119.001507⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9659679d7afa299fbc4bde076b980de0 https://www.hal.inserm.fr/inserm-02941608 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9659679d7afa299fbc4bde076b980de0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15359476 15359484 |
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| DOI: | 10.1074/mcp.RA119.001507⟩ |