Nonhuman Primate Models for Diabetic Ocular Neovascularization Using AAV2-Mediated Overexpression of Vascular Endothelial Growth Factor
| العنوان: | Nonhuman Primate Models for Diabetic Ocular Neovascularization Using AAV2-Mediated Overexpression of Vascular Endothelial Growth Factor |
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| المؤلفون: | Corinna Lebherz, Rebecca Grant, Alberto Auricchio, Waixing Tang, James M. Wilson, Artur V. Cideciyan, Albert M. Maguire, Tomas S. Aleman, Jean Bennett, Samuel G. Jacobson, Zhangyong Wei |
| المصدر: | Diabetes. 54:1141-1149 |
| بيانات النشر: | American Diabetes Association, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Vascular Endothelial Growth Factor A, medicine.medical_specialty, Time Factors, genetic structures, Endocrinology, Diabetes and Metabolism, Gene Expression, Retinal Neovascularization, Transfection, Retina, Adenoviridae, Neovascularization, chemistry.chemical_compound, Ophthalmology, Internal Medicine, medicine, Animals, Diabetic Retinopathy, business.industry, Retinopathy of prematurity, Diabetic retinopathy, Dependovirus, Macular degeneration, medicine.disease, Macaca mulatta, eye diseases, Surgery, Vascular endothelial growth factor, Disease Models, Animal, Vascular endothelial growth factor A, Choroidal neovascularization, chemistry, Intraocular fluid, Viruses, Female, sense organs, medicine.symptom, business |
| الوصف: | Neovascularization leads to blindness in numerous ocular diseases, including diabetic retinopathy, age-related macular degeneration, retinopathy of prematurity, and sickle cell disease. More effective and stable treatments for ocular neovascularization are needed, yet there are major limitations in the present animal models. To develop primate models of diabetic retinopathy and choroidal neovascularization, rhesus monkeys were injected subretinally or intravitreally with an adeno-associated virus (AAV)-2 vector carrying the cDNA encoding human vascular endothelial growth factor (VEGF). Overexpression of VEGF was measured by intraocular fluid sampling over time. Neovascularization was evaluated by ophthalmoscopy through angiography, optical coherence tomography, and ultimately histopathology. Overexpression of VEGF through AAV2 results in rapid development of features of diabetic retinopathy or macular edema, depending on the targeted cell type/mode of production of VEGF and diffusion of VEGF. Nonhuman primate models will be useful in testing long-term safety and efficacy of novel therapeutic agents for blinding neovascular diseases. |
| تدمد: | 1939-327X 0012-1797 |
| DOI: | 10.2337/diabetes.54.4.1141 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95240559bd10d1fcabaf2fc773c54185 https://doi.org/10.2337/diabetes.54.4.1141 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....95240559bd10d1fcabaf2fc773c54185 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1939327X 00121797 |
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| DOI: | 10.2337/diabetes.54.4.1141 |