A triple suicide gene strategy that improves therapeutic effects and incorporates multi-modality molecular imaging for monitoring gene-functions
| العنوان: | A triple suicide gene strategy that improves therapeutic effects and incorporates multi-modality molecular imaging for monitoring gene-functions |
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| المؤلفون: | Jason A. Koutcher, Ellen Ackerstaff, Khushali Kotedia, Gloria C. Li, C. Clifton Ling, Ligang Xing, Xuelong Deng, Pat Zanzonico, Xiaorong Sun |
| المصدر: | Cancer gene therapy |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Cancer Research, Magnetic Resonance Spectroscopy, magnetic resonance-spectroscopy, Biology, Transfection, Thymidine Kinase, Article, positron-emission-tomography, Gene-directed enzyme prodrug therapy, Cytosine Deaminase, 03 medical and health sciences, optical imaging, 0302 clinical medicine, In vivo, Cell Line, Tumor, Neoplasms, Humans, Prodrugs, Pentosyltransferases, Molecular Biology, 030304 developmental biology, 0303 health sciences, Uracil phosphoribosyltransferase, Clinical Trials as Topic, Radiotherapy, Cytosine deaminase, Genes, Transgenic, Suicide, Genetic Therapy, Prodrug, Suicide gene, Molecular biology, 3. Good health, Thymidine kinase, 030220 oncology & carcinogenesis, Positron-Emission Tomography, Cancer research, Molecular Medicine, Molecular imaging |
| الوصف: | Gene-directed enzyme prodrug therapy (GDEPT), or suicide gene therapy, has shown promise in clinical trials. In this preclinical study using stable cell lines and xenograft tumor models, we show that a triple-suicide-gene GDEPT approach produce enhanced therapeutic efficacy over previous methods. Importantly all the three genes (thymidine kinase, cytosine deaminase, and uracil phosphoribosyltransferase) function simultaneously as effectors for GDEPT and markers for multimodality molecular imaging (MMI), using positron-emission-tomography (PET), magnetic resonance-spectroscopy (MRS), and optical (fluorescent and bioluminescent) techniques. It was demonstrated that MMI can evaluate the distribution and function/activity of the triple-suicide-gene. The concomitant expression of these genes significantly enhances prodrug cytotoxicity and radiosensitivity in vitro and in vivo. |
| اللغة: | English |
| تدمد: | 1476-5500 0929-1903 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93d96ce2c056bde05b997a40e247044f http://europepmc.org/articles/PMC3696018 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....93d96ce2c056bde05b997a40e247044f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765500 09291903 |
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