Differentiation of symbiotic cells and endosymbionts in Medicago truncatula nodulation are coupled to two transcriptome-switches
العنوان: | Differentiation of symbiotic cells and endosymbionts in Medicago truncatula nodulation are coupled to two transcriptome-switches |
---|---|
المؤلفون: | Nicolas Agier, Miguel Redondo-Nieto, Willem Van de Velde, Pascal Ratet, Marie Bourcy, Laetitia Marisa, Daniele Vaubert, Gérard Duc, Nicolas Maunoury, Lawrence P. Aggerbeck, Hervé Delacroix, Patricia Durand, Philippe Laporte, Peter Mergaert, Benoit Alunni, Eva Kondorosi |
المساهمون: | Centre National de la Recherche Scientifique (CNRS), DNA MicroArray Platform (GODMAP), Partenaires INRAE, Université Paris-Sud - Paris 11 (UP11), UMR 0102 - Unité de Recherche Génétique et Ecophysiologie des Légumineuses, Génétique et Ecophysiologie des Légumineuses à Graines (UMRLEG) (UMR 102), Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Bay Zoltan Foundation for Applied Research |
المصدر: | Plos One 3 (5), 1-14. (2010) PLoS ONE, Vol 5, Iss 3, p e9519 (2010) PLoS ONE PLoS ONE, Public Library of Science, 2010, 5 (3), pp.1-14. ⟨10.1371/journal.pone.0009519⟩ |
سنة النشر: | 2010 |
مصطلحات موضوعية: | 0106 biological sciences, Root nodule, Cellular differentiation, lcsh:Medicine, 01 natural sciences, Genetics and Genomics/Plant Genetics and Gene Expression, Transcriptome, CYTOLOGY, rhizobium, Gene Expression Regulation, Plant, Medicago, SYMBIOTIC MUTANT, lcsh:Science, Expressed Sequence Tags, 2. Zero hunger, 0303 health sciences, Sinorhizobium meliloti, Multidisciplinary, biology, food and beverages, Cell Differentiation, medicago truncatula, Medicago truncatula, Cell biology, Phenotype, medicine.symptom, symbiose, Plant Biology/Plant Cell Biology, Algorithms, Research Article, Plant Biology/Plant-Biotic Interactions, Genetic Markers, Nitrogen, NOD+, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Rhizobia, 03 medical and health sciences, FIX, Nitrogen Fixation, Botany, medicine, Symbiosis, 030304 developmental biology, Ploidies, Gene Expression Profiling, lcsh:R, Nodule (medicine), Gene Expression Regulation, Bacterial, biology.organism_classification, Plant cell, Mutation, lcsh:Q, transcriptome, 010606 plant biology & botany |
الوصف: | International audience; The legume plant Medicago truncatula establishes a symbiosis with the nitrogen-fixing bacterium Sinorhizobium meliloti which takes place in root nodules. The formation of nodules employs a complex developmental program involving organogenesis, specific cellular differentiation of the host cells and the endosymbiotic bacteria, called bacteroids, as well as the specific activation of a large number of plant genes. By using a collection of plant and bacterial mutants inducing non-functional, Fix− nodules, we studied the differentiation processes of the symbiotic partners together with the nodule transcriptome, with the aim of unravelling links between cell differentiation and transcriptome activation. Two waves of transcriptional reprogramming involving the repression and the massive induction of hundreds of genes were observed during wild-type nodule formation. The dominant features of this “nodule-specific transcriptome” were the repression of plant defense-related genes, the transient activation of cell cycle and protein synthesis genes at the early stage of nodule development and the activation of the secretory pathway along with a large number of transmembrane and secretory proteins or peptides throughout organogenesis. The fifteen plant and bacterial mutants that were analyzed fell into four major categories. Members of the first category of mutants formed non-functional nodules although they had differentiated nodule cells and bacteroids. This group passed the two transcriptome switch-points similarly to the wild type. The second category, which formed nodules in which the plant cells were differentiated and infected but the bacteroids did not differentiate, passed the first transcriptome switch but not the second one. Nodules in the third category contained infection threads but were devoid of differentiated symbiotic cells and displayed a root-like transcriptome. Nodules in the fourth category were free of bacteria, devoid of differentiated symbiotic cells and also displayed a root-like transcriptome. A correlation thus exists between the differentiation of symbiotic nodule cells and the first wave of nodule specific gene activation and between differentiation of rhizobia to bacteroids and the second transcriptome wave in nodules. The differentiation of symbiotic cells and of bacteroids may therefore constitute signals for the execution of these transcriptome-switches. |
وصف الملف: | application/pdf |
اللغة: | English |
تدمد: | 1932-6203 |
DOI: | 10.1371/journal.pone.0009519⟩ |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::932ee89eaf54271ad91e294d465a2a4b http://prodinra.inra.fr/record/36396 |
Rights: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....932ee89eaf54271ad91e294d465a2a4b |
قاعدة البيانات: | OpenAIRE |
تدمد: | 19326203 |
---|---|
DOI: | 10.1371/journal.pone.0009519⟩ |