Dendritic cells transmit HIV-1 through human small intestinal mucosa
| العنوان: | Dendritic cells transmit HIV-1 through human small intestinal mucosa |
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| المؤلفون: | Lea Novak, Ruizhong Shen, Lesley E. Smythies, Ronald H. Clements, Phillip D. Smith |
| المصدر: | Journal of Leukocyte Biology. 87:663-670 |
| بيانات النشر: | Oxford University Press (OUP), 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Receptors, CCR4, Receptors, CCR5, T-Lymphocytes, Immunology, chemical and pharmacologic phenomena, HIV Infections, Receptors, Cell Surface, C-C chemokine receptor type 7, Biology, CXCR4, Jejunum, Intestinal mucosa, Antigens, CD, medicine, Humans, Immunology and Allergy, Lectins, C-Type, Intestinal Mucosa, Receptor, Cells, Cultured, CD86, Lamina propria, virus diseases, hemic and immune systems, Dendritic Cells, Cell Biology, Epithelium, Cell biology, medicine.anatomical_structure, HIV-1, Primary Research, Cell Adhesion Molecules |
| الوصف: | Human intestinal dendritic cells take up, transport, and transmit HIV-1 to lamina propria and peripheral blood lymphocytes. To dissect the early events in the transmission of HIV-1 from mother to child, we investigated whether DCs participate in HIV-1 entry into human small intestinal mucosa. We isolated human MNLs from jejunal lamina propria and identified a subpopulation of CD11c+HLA-DR+ MNLs that expressed DC-SIGN, CD83, CD86, CD206, and CCR7, indicating a DC phenotype. Jejunal DCs also expressed the HIV-1 receptor CD4 and coreceptors CCR5 and CXCR4 and in suspension rapidly took up cell-free HIV-1. HIV-1 inoculated onto the apical surface of explanted jejunum was transported by lamina propria DCs through the mucosa and transmitted in trans to blood and intestinal lymphocytes. These findings indicate that in addition to intestinal epithelial cells, which we showed previously transcytose infectious HIV-1 to indicator cells, intestinal DCs play an important role in transporting HIV-1 through the intestinal mucosa and the subsequent transmission to T cells. |
| تدمد: | 1938-3673 0741-5400 |
| DOI: | 10.1189/jlb.0909605 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::914829c2be8b52e3c5fa0574ba3cb558 https://doi.org/10.1189/jlb.0909605 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....914829c2be8b52e3c5fa0574ba3cb558 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19383673 07415400 |
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| DOI: | 10.1189/jlb.0909605 |