henn-1/HEN1 Promotes Germline Immortality in Caenorhabditis elegans
| العنوان: | henn-1/HEN1 Promotes Germline Immortality in Caenorhabditis elegans |
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| المؤلفون: | Taylor N Marks, Kailee J. Reed, Brooke E Montgomery, Tarah Vijayasarathy, Dieu An H. Nguyen, Rachel M. Tucci, Carolyn M. Phillips, Kristen C. Brown, Taiowa A. Montgomery, Joshua M. Svendsen |
| المصدر: | Cell reports Cell Reports, Vol 29, Iss 10, Pp 3187-3199.e4 (2019) |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Small RNA, endocrine system, Piwi-interacting RNA, Nerve Tissue Proteins, Methylation, General Biochemistry, Genetics and Molecular Biology, Germline, Article, 03 medical and health sciences, 0302 clinical medicine, RNA interference, microRNA, Animals, Epigenetics, Gene Silencing, RNA, Small Interfering, Caenorhabditis elegans, Caenorhabditis elegans Proteins, lcsh:QH301-705.5, Cell Proliferation, biology, urogenital system, Methyltransferases, Argonaute, biology.organism_classification, Cell biology, 030104 developmental biology, Germ Cells, lcsh:Biology (General), 030217 neurology & neurosurgery |
| الوصف: | SUMMARY The germline contains an immortal cell lineage that ensures the faithful transmission of genetic and, in some instances, epigenetic information from one generation to the next. Here, we show that in Caenorhabditis elegans, the small RNA 3′-2′-O-methyltransferase henn-1/HEN1 is required for sustained fertility across generations. In the absence of henn-1, animals become progressively less fertile, becoming sterile after ~30 generations at 25°C. Sterility in henn-1 mutants is accompanied by severe defects in germline proliferation and maintenance. The requirement for henn-1 in transgenerational fertility is likely due to its role in methylating and, thereby, stabilizing Piwi-interacting RNAs (piRNAs). However, despite being essential for piRNA stability in embryos, henn-1 is not required for piRNA stability in adults. Thus, we propose that methylation is important for the role of piRNAs in establishing proper gene silencing during early stages of development but is dispensable for their role in the proliferated germline. In Brief Svendsen et al. identify a requirement for the small RNA methyltransferase HENN-1 in germline immortality. HENN-1 is required for piRNA stability during embryogenesis but is dispensable in the adult germline, pointing to a role for piRNAs in establishing a gene regulatory network in embryos that protects the germline throughout development. Graphical Abstract |
| تدمد: | 2211-1247 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90df4b9487ca9895181c654ef9dff6b3 https://pubmed.ncbi.nlm.nih.gov/31801082 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....90df4b9487ca9895181c654ef9dff6b3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22111247 |
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