Development of a new LC-MS method for accurate and sensitive determination of 33 pyrrolizidine and 21 tropane alkaloids in plant-based food matrices

التفاصيل البيبلوغرافية
العنوان: Development of a new LC-MS method for accurate and sensitive determination of 33 pyrrolizidine and 21 tropane alkaloids in plant-based food matrices
المؤلفون: Zbynek Dzuman, Milena Stranska-Zachariasova, Jana Hajslova, Marie Fenclova, Petra Jonatova, Alena Novakova, Ondrej Brabenec, Nela Prusova
المصدر: Analytical and Bioanalytical Chemistry. 412:7155-7167
بيانات النشر: Springer Science and Business Media LLC, 2020.
سنة النشر: 2020
مصطلحات موضوعية: Analyte, Food Contamination, 02 engineering and technology, Tandem mass spectrometry, 01 natural sciences, Biochemistry, Analytical Chemistry, chemistry.chemical_compound, Herbal tea, Alkaloids, Isomerism, Limit of Detection, Tandem Mass Spectrometry, Liquid chromatography–mass spectrometry, Chromatography, High Pressure Liquid, Pyrrolizidine Alkaloids, Chromatography, Hydrophilic interaction chromatography, 010401 analytical chemistry, Reproducibility of Results, Tropane, Plants, 021001 nanoscience & nanotechnology, 0104 chemical sciences, chemistry, Pyrrolizidine, 0210 nano-technology, Selectivity, Tropanes
الوصف: Setting of maximum limits for a number of plant alkaloids is under discussion in the EU. The novel method developed and optimized in this study enables simultaneous determination of 21 tropane alkaloids (TAs) and 33 pyrrolizidine (PAs) together with their N-oxides (PANOs). For analysis of aqueous-methanolic extract, reversed phase ultra-high-performance liquid chromatography and tandem mass spectrometry (RP-U-HPLC-MS/MS) was employed. The method was validated for frequently contaminated matrices (i) sorghum, (ii) oregano, and (iii) mixed herbal tea. The recoveries at two spiking levels were in the range of 82–115%, 80–106%, and 78–117%, respectively, and repeatabilities were less than 19% for all analyte/matrix combinations. As regards the achieved limits of quantification (LOQ), their values were in the range of 0.5–10 μg kg−1. The crucial problem encountered during method development, co-elution of multiple groups of isomeric alkaloids, was overcome by subsequent sample separation in the second chromatographic system, hydrophilic interaction liquid chromatography (HILIC), providing different separation selectivity. Lycopsamine, echinatine, and indicine (co-elution group 1) and N-oxides of indicine and intermedine (co-elution group 2), which could not be resolved on the commonly used RP column, were possible to separate fully by using the HILIC system.
تدمد: 1618-2650
1618-2642
DOI: 10.1007/s00216-020-02848-6
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90acb9a93e891f8e8bc026a36c741ff5
https://doi.org/10.1007/s00216-020-02848-6
Rights: CLOSED
رقم الانضمام: edsair.doi.dedup.....90acb9a93e891f8e8bc026a36c741ff5
قاعدة البيانات: OpenAIRE
الوصف
تدمد:16182650
16182642
DOI:10.1007/s00216-020-02848-6