Association between dense CADM1 promoter methylation and reduced protein expression in high-grade CIN and cervical SCC
| العنوان: | Association between dense CADM1 promoter methylation and reduced protein expression in high-grade CIN and cervical SCC |
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| المؤلفون: | Renske D.M. Steenbergen, Saskia M. Wilting, Peter J.F. Snijders, Florianne E. Henken, Akihiko Ito, Johannes Berkhof, Daniëlle A.M. Heideman, René Overmeer, Yoshinori Murakami, Theo J.M. Helmerhorst, Chris J.L.M. Meijer, Debbie Claassen-Kramer |
| المساهمون: | Obstetrics & Gynecology, Immunology, Pathology, Epidemiology and Data Science, CCA - Disease profiling |
| المصدر: | Journal of Pathology, 215(4), 388-397. John Wiley & Sons Ltd. Journal of Pathology, 215(4), 388-397. John Wiley and Sons Ltd Overmeer, R M, Henken, F E, Snijders, P J F, Claassen-Kramer, D, Berkhof, J, Helmerhorst, TJ, Heideman, D A M, Wilting, S M, Murakami, Y, Ito, A, Meijer, C J L M & Steenbergen, R D M 2008, ' Association between dense CADM1 promoter methylation and reduced protein expression in high-grade CIN and cervical SCC ', Journal of Pathology, vol. 215, no. 4, pp. 388-397 . https://doi.org/10.1002/path.2367 |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, Tumor suppressor gene, Blotting, Western, Immunoglobulins, Uterine Cervical Neoplasms, Biology, Cervical intraepithelial neoplasia, Pathology and Forensic Medicine, SDG 3 - Good Health and Well-being, Biomarkers, Tumor, medicine, Humans, Gene silencing, Gene Silencing, Promoter Regions, Genetic, Cell Line, Transformed, Cervical cancer, Reverse Transcriptase Polymerase Chain Reaction, Tumor Suppressor Proteins, Cell Adhesion Molecule-1, Membrane Proteins, Cancer, Methylation, DNA Methylation, Uterine Cervical Dysplasia, medicine.disease, Immunohistochemistry, Logistic Models, Case-Control Studies, DNA methylation, Carcinoma, Squamous Cell, Cancer research, Female, Cell Adhesion Molecules |
| الوصف: | We previously showed that silencing of TSLC1 recently renamed CADM1, is functionally involved in high-risk HPV-mediated cervical carcinogenesis. CADM1 silencing often results from promoter methylation. Here, we determined the extent of CADM1 promoter methylation in cervical (pre)malignant lesions and its relation to anchorage-independent growth and gene silencing to select a CADM1-based methylation marker for identification of women at risk of cervical cancer. Methylation-specific PCRs targeting three regions within the CADM1 promoter were performed on high-risk HPV-containing cell lines, PBMCs, normal cervical smears, and (pre)malignant lesions. CADM1 protein expression in cervical tissues was analysed by immunohistochemistry. All statistical tests were two-sided. Density of methylation was associated with the degree of anchorage-independent growth and CADM1 gene silencing in vitro. In cervical squamous lesions, methylation frequency and density increased with severity of disease. Dense methylation (defined as >= 2 methylated regions) increased from 5% in normal cervical samples to 30% in CIN3 lesions and 83% in squamous cell carcinomas (SCCs) and was significantly associated with decreased CADM1 protein expression (p < 0.00005). The frequency of dense methylation was significantly higher in >= CIN3 compared with = CIN3. Copyright (c) 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. |
| تدمد: | 0022-3417 |
| DOI: | 10.1002/path.2367 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e614da7ecc809d78a02fb0bf22509fb https://doi.org/10.1002/path.2367 |
| Rights: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....8e614da7ecc809d78a02fb0bf22509fb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00223417 |
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| DOI: | 10.1002/path.2367 |