Apoptosis Sensitization by Euphorbia Factor L1 in ABCB1-Mediated Multidrug Resistant K562/ADR Cells
| العنوان: | Apoptosis Sensitization by Euphorbia Factor L1 in ABCB1-Mediated Multidrug Resistant K562/ADR Cells |
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| المؤلفون: | Yi-Na Tang, Xicheng He, Min-Ting Lin, Tao Yi, Lan-Lan Fan, Jianye Zhang, Hubiao Chen, Zhongzhen Zhao |
| المصدر: | Molecules Molecules; Volume 18; Issue 10; Pages: 12793-12808 Molecules, Vol 18, Iss 10, Pp 12793-12808 (2013) |
| بيانات النشر: | MDPI, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | MAPK/ERK pathway, Pharmaceutical Science, Gene Expression, Apoptosis, Pharmacology, Rhodamine 123, Analytical Chemistry, chemistry.chemical_compound, Euphorbia factor L1, Annexin, hemic and lymphatic diseases, Drug Discovery, Phosphorylation, Extracellular Signal-Regulated MAP Kinases, Sensitization, medicine.diagnostic_test, Phenylpropionates, ABCB1, Drug Synergism, Drug Resistance, Multiple, medicine.anatomical_structure, Chemistry (miscellaneous), Vincristine, Caspases, Molecular Medicine, Diterpenes, ATP Binding Cassette Transporter, Subfamily B, Cell Survival, chemical and pharmacologic phenomena, Antineoplastic Agents, Biology, Article, lcsh:QD241-441, Inhibitory Concentration 50, lcsh:Organic chemistry, Western blot, multidrug resistance, medicine, Humans, ATP Binding Cassette Transporter, Subfamily B, Member 1, Physical and Theoretical Chemistry, Protein kinase B, Organic Chemistry, chemistry, Doxorubicin, Drug Resistance, Neoplasm, Cancer research, apoptosis, Drug Screening Assays, Antitumor, K562 Cells, Protein Processing, Post-Translational, Proto-Oncogene Proteins c-akt, K562 cells |
| الوصف: | In this article, reversal activities of Euphorbia factor L1 (EFL1) against ABCB1-mediated multidrug resistance (MDR) and apoptosis sensitization in K562/ADR cells are reported. EFL1 decreased the IC50 values of anticancer agents in K562/ADR cells over-expressing ABCB1. However, EFL1 did not affect the IC50 values of anticancer agents in sensitive K562 cells. Additionally, EFL1 increased the intracellular accumulation of rhodamine 123 and doxorubicin in K562/ADR cells without affecting their accumulation in K562 cells. Furthermore, EFL1 sensitized the apoptosis triggered by vincristine in K562/ADR cells via mitochondrial pathway, as confirmed by Annexin V-FITC/PI detection and western blot. At the same time, EFL1 did not influence the apoptosis induced by vincristine in K562 cells. Western blot results showed that EFL1 did not affect the phosphorylation level of AKT and ERK in K562 and K562/ADR cells. Finally, EFL1 did not down-regulate protein expression of ABCB1. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1420-3049 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8dc53792ca89478c15dc8838dab84a35 http://europepmc.org/articles/PMC6270536 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8dc53792ca89478c15dc8838dab84a35 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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