Lipid signature of advanced human carotid atherosclerosis assessed by mass spectrometry imaging
| العنوان: | Lipid signature of advanced human carotid atherosclerosis assessed by mass spectrometry imaging |
|---|---|
| المؤلفون: | Antonius Fw van der Steen, Hence J.M. Verhagen, Mirjam Visscher, Nuria Slijkhuis, Peter C. Burgers, Heleen M.M. van Beusekom, Theo Klein, Astrid M. Moerman, Kim Van der Heiden, Yolanda B. de Rijke, Kim van Gaalen, Gijs van Soest, Bram Heijs, Theo M. Luider, Frank J. H. Gijsen |
| المساهمون: | Cardiology, Clinical Chemistry, Neurology, Surgery |
| المصدر: | Journal of Lipid Research, Vol 62, Iss, Pp 100020-(2021) Journal of Lipid Research Journal of Lipid Research, 62. ELSEVIER Journal of Lipid Research, 62:100020. American Society for Biochemistry and Molecular Biology Inc. |
| بيانات النشر: | ELSEVIER, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Carotid Artery Diseases, 0301 basic medicine, Pathology, medicine.medical_treatment, FC, foam cells, Carotid endarterectomy, 030204 cardiovascular system & hematology, NMF, nonnegative matrix factorization, Biochemistry, PC, phosphatidylcholine, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, DG, diacylglycerol, mass spectrometry, education.field_of_study, CE, cholesteryl ester, imaging, MSI, mass spectrometry imaging, DCM, dichloromethane, TG, triacylglycerol, Cholesteryl ester, lipids (amino acids, peptides, and proteins), Sphingomyelin, Research Article, medicine.medical_specialty, Population, QD415-436, Mass spectrometry imaging, sphingomyelin, lipids, histology, 03 medical and health sciences, NC, necrotic core, SDG 3 - Good Health and Well-being, Vascular Biology, oxidized lipids, Lipidomics, medicine, ischemic stroke, education, LPC, lysophosphatidylcholine, SM, sphingomyelin, sphingolipids, Colocalization, Cell Biology, VIP, variable influence on projection, Sphingolipid, 030104 developmental biology, CEA, carotid endarterectomy, chemistry, OPLS-DA, orthogonal projections to latent structures discriminant analysis, lipidomics, atherosclerosis |
| الوصف: | Carotid atherosclerosis is a risk factor for ischemic stroke, one of the main causes of mortality and disability worldwide. The disease is characterized by plaques, heterogeneous deposits of lipids, and necrotic debris in the vascular wall, which grow gradually and may remain asymptomatic for decades. However, at some point a plaque can evolve to a high-risk plaque phenotype, which may trigger a cerebrovascular event. Lipids play a key role in the development and progression of atherosclerosis, but the nature of their involvement is not fully understood. Using matrix-assisted laser desorption/ionization mass spectrometry imaging, we visualized the distribution of approximately 200 different lipid signals, originating of >90 uniquely assigned species, in 106 tissue sections of 12 human carotid atherosclerotic plaques. We performed unsupervised classification of the mass spectrometry dataset, as well as a histology-directed multivariate analysis. These data allowed us to extract the spatial lipid patterns associated with morphological plaque features in advanced plaques from a symptomatic population, revealing spatial lipid patterns in atherosclerosis and their relation to histological tissue type. The abundances of sphingomyelin and oxidized cholesteryl ester species were elevated specifically in necrotic intima areas, whereas diacylglycerols and triacylglycerols were spatially correlated to areas containing the coagulation protein fibrin. These results demonstrate a clear colocalization between plaque features and specific lipid classes, as well as individual lipid species in high-risk atherosclerotic plaques. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0022-2275 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d440cac86ce846438ba8fc6a1578790 https://hdl.handle.net/1887/3246770 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8d440cac86ce846438ba8fc6a1578790 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00222275 |
|---|