Interlaboratory analysis of teicoplanin plasma concentration assays among Chinese laboratories
| العنوان: | Interlaboratory analysis of teicoplanin plasma concentration assays among Chinese laboratories |
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| المؤلفون: | Wenqian Chen, Wei Qin, Pengmei Li, Xianglin Zhang, Xu‐Dong Kong, Wang-Jun Qin, Shuo Xu, Peng‐Fei Jin, Xiaoxue Wang |
| المصدر: | Journal of Clinical Pharmacy and Therapeutics. 45:983-990 |
| بيانات النشر: | Hindawi Limited, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, Quality Control, China, Adolescent, 030226 pharmacology & pharmacy, High-performance liquid chromatography, Young Adult, 03 medical and health sciences, 0302 clinical medicine, medicine, Humans, Pharmacology (medical), 030212 general & internal medicine, Hplc method, Chromatography, High Pressure Liquid, Aged, Aged, 80 and over, Pharmacology, Chromatography, medicine.diagnostic_test, business.industry, Teicoplanin, Quality assessment, Reproducibility of Results, Middle Aged, Anti-Bacterial Agents, Clinical Practice, Therapeutic drug monitoring, Plasma concentration, Drug Monitoring, Laboratories, business, medicine.drug |
| الوصف: | What is known and objective Teicoplanin is widely used for the treatment of infections caused by drug-resistant Gram-positive bacteria. Since there is a good correlation between trough levels and clinical outcome, therapeutic drug monitoring (TDM) is recommended to achieve better clinical curative effects. However, TDM of teicoplanin is not routine in China. So, a programme was initiated in 2017, including both HPLC method establishment and interlaboratory quality assessment, for the measurement of teicoplanin. Methods A main centre and a quality control centre were set up in the study. An HPLC-based method of teicoplanin determination in plasma was developed by the main centre. Analysis was performed using a Waters Symmetry C18 column (250 mm × 4.6 mm, 5 µm). The mobile phase was NaH2 PO4 (0.01 mol/L) and acetonitrile (75:25 v/v; pH 3.3), with a flow rate of 1.0 mL/min and a detection wavelength of 215 nm. Piperacillin sodium was selected as an internal standard (IS). Twenty-six additional TDM centres were then recruited to adopt this method. Then, all the centres were asked to take part in a quality control assessment evaluated by the quality control centre. Results For all TDM centres, linearity of teicoplanin concentration ranges was between 3.125 and 100 µg/mL. Intraday and interday accuracies ranged from 87.1% to 118.4%. Intraday and interday precision ranged from 0.3% to 13.8%. Therapeutic drug monitoring centres all passed inter-room quality assessment. All samples tested met the acceptance criteria. Then, 542 samples were collected. Patients with sub-optimal (≤10 mg/L) plasma teicoplanin concentrations constituted 42% of the total study population. What is new and conclusions For the first time, a simple, rapid and accurate HPLC method for determining teicoplanin levels was successfully applied to therapeutic drug monitoring in clinical practice for twenty-seven TDM centres in China. The results demonstrated excellent interlaboratory agreement for teicoplanin testing and provide support for clinical laboratory quality management and results inter-accreditation. |
| تدمد: | 1365-2710 0269-4727 |
| DOI: | 10.1111/jcpt.13115 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d2bf99d091328b1b45f9a2522b4fe73 https://doi.org/10.1111/jcpt.13115 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....8d2bf99d091328b1b45f9a2522b4fe73 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652710 02694727 |
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| DOI: | 10.1111/jcpt.13115 |