A Mechanism for TCR Sharing between T Cell Subsets and Individuals Revealed by Pyrosequencing
| العنوان: | A Mechanism for TCR Sharing between T Cell Subsets and Individuals Revealed by Pyrosequencing |
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| المؤلفون: | Jorge R. Almeida, Hui Yee Greenaway, Pauline C. Ng, Daniel C. Douek, Máire F. Quigley, Vanessa Venturi, David Price, Tedi E. Asher, Zachary Ende, Miles P. Davenport, Tina C McIntosh, Samuel Levy |
| المصدر: | The Journal of Immunology. 186:4285-4294 |
| بيانات النشر: | The American Association of Immunologists, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Adult, Male, Naive T cell, Receptors, Antigen, T-Cell, alpha-beta, T cell, Immunology, Immunoglobulin Variable Region, Biology, Resting Phase, Cell Cycle, Deep sequencing, Young Adult, T-Lymphocyte Subsets, medicine, Humans, Immunology and Allergy, Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, Recombination, Genetic, Genetics, Repertoire, T-cell receptor, High-Throughput Nucleotide Sequencing, Gene rearrangement, Middle Aged, Phenotype, Clone Cells, medicine.anatomical_structure, Evolutionary biology, Immunologic Memory, CD8 |
| الوصف: | The human naive T cell repertoire is the repository of a vast array of TCRs. However, the factors that shape their hierarchical distribution and relationship with the memory repertoire remain poorly understood. In this study, we used polychromatic flow cytometry to isolate highly pure memory and naive CD8+ T cells, stringently defined with multiple phenotypic markers, and used deep sequencing to characterize corresponding portions of their respective TCR repertoires from four individuals. The extent of interindividual TCR sharing and the overlap between the memory and naive compartments within individuals were determined by TCR clonotype frequencies, such that higher-frequency clonotypes were more commonly shared between compartments and individuals. TCR clonotype frequencies were, in turn, predicted by the efficiency of their production during V(D)J recombination. Thus, convergent recombination shapes the TCR repertoire of the memory and naive T cell pools, as well as their interrelationship within and between individuals. |
| تدمد: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.1003898 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a107c14f0f06d1aa5749d3143fa8c7d https://doi.org/10.4049/jimmunol.1003898 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8a107c14f0f06d1aa5749d3143fa8c7d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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| DOI: | 10.4049/jimmunol.1003898 |