Molecular identification of Candida auris by PCR amplification of species-specific GPI protein-encoding genes
| العنوان: | Molecular identification of Candida auris by PCR amplification of species-specific GPI protein-encoding genes |
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| المؤلفون: | Piet W. J. de Groot, Eulogio Valentín, María Ángeles Tormo-Más, Elena Eraso, Jordan Fernández-Pereira, Alba Ruiz-Gaitán, Javier Pemán |
| المصدر: | INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe instname |
| بيانات النشر: | Elsevier BV, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), 030106 microbiology, Biology, Polymerase Chain Reaction, Microbiology, Disease Outbreaks, law.invention, 03 medical and health sciences, law, Drug Resistance, Multiple, Fungal, Humans, Gene, Polymerase chain reaction, Candida, Molecular identification, Colony PCR, Base Sequence, Candidiasis, Glucose-6-Phosphate Isomerase, Outbreak, General Medicine, Gpi anchored protein, GPI protein, Invasive candidiasis, Infectious Diseases, Candida auris, Spain, C. auris, Bloodstream infections, Primer (molecular biology), Variants of PCR, Nucleic Acid Amplification Techniques |
| الوصف: | The emerging multidrug-resistant pathogenic yeast Candida auris causes life-threatening invasive infections and shows a capacity for hospital transmission that is uncommon in other Candida species. Rapid and accurate diagnosis of C. curls infections is crucial; however, the fungus is frequently misidentified. Here, we present a rapid and easily applicable PCR assay for reliable identification of C. auris by designing primers from unique GPI protein-encoding genes. Specificity of the used primers for C. auris was verified with a panel of 19 different Candida species including the clinically most relevant and phylogenetically closely related species. Efficacy of the PCR approach was validated by correctly identifying 112 C. auris isolates from an outbreak in a Spanish hospital, 20% of which were not reliably identified by MALDI-TOF MS, and 27 genotypically diverse C. auris isolates originating from hospitals in various countries, in a test that included (blind) negative controls. By employing two GPI protein primer pairs in a single PCR, a double screening can be performed, which enhances the robustness of the PCR assay and avoids potential false negatives due to recent evolutionary events, as was observed for two isolates. Our PCR method, which is based on the uniqueness of selected GPI protein-encoding genes, is useful for easy, low-cost, and accurate identification of C. auris infections in a clinical setting. |
| تدمد: | 1438-4221 |
| DOI: | 10.1016/j.ijmm.2018.06.014 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8944871dbaefa84fcd7a44e702727984 https://doi.org/10.1016/j.ijmm.2018.06.014 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8944871dbaefa84fcd7a44e702727984 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14384221 |
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| DOI: | 10.1016/j.ijmm.2018.06.014 |