Oncogenic microRNA‐765 promotes the growth and metastasis of breast carcinoma by directly targeting ING4
| العنوان: | Oncogenic microRNA‐765 promotes the growth and metastasis of breast carcinoma by directly targeting ING4 |
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| المؤلفون: | Xiao-Mei Li, Junhua Yu, Hong-Xia Wu, Yan-Yan Jiao, Cailing Yuan |
| المصدر: | Journal of Cellular Biochemistry. 121:3887-3900 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Cell growth, Cell, Cell Biology, Transfection, Biology, medicine.disease, Biochemistry, Metastasis, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, Breast cancer, Downregulation and upregulation, 030220 oncology & carcinogenesis, microRNA, medicine, Cancer research, Breast carcinoma, Molecular Biology |
| الوصف: | Previous investigations have proved that microRNA (miR)-765 is significantly overexpressed in multiple tumor types. Nevertheless, the underlying molecular mechanism of miR-765 in mediating breast carcinoma cell growth and metastasis remains unclear. Quantitative real-time polymerase chain reaction was used to determine the levels of miR-765 and inhibitor of growth 4 (ING4) in breast carcinoma tissues and breast carcinoma cells. Cell proliferation, colony formation, wound healing, and Transwell invasion assays were used to analysis the role of miR-765 on breast carcinoma cell growth and aggressiveness. The expressions of ING4 were determined using Western blot analysis and immunohistochemical staining. The direct target of ING4 and miR-765 was confirmed using the luciferase reporter assay. Nude mice were subcutaneously implanted with miR-765 inhibitor transfected MDA-MB-231 cells to determine the potential role of miR-765 in tumor growth in vivo. We observed that miR-765 is overexpressed in breast carcinoma tissue and breast cancer cells. By using luciferase reporter gene bioassay, we find that ING4 is the direct target of miR-765 in breast carcinoma. The level of ING4 is inversely associated with the level of miR-765. The gain-of-function and loss-of-function experiments in vitro indicate that the downregulation of miR-765 suppresses the growth, mobility, and invasion abilities of breast cancer cells by inhibiting ING4. In addition, overexpression of ING4 suppresses the aggressiveness of the MDA-BA-231 cell that is induced by miR-761 in vitro. In this study, we prove that miR-765 regulates the growth and metastasis of breast cancer via modulating miR-765-ING4-negative feedback loop. |
| تدمد: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.29545 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87c0ea22f3d8fcf49be56c448354e71a https://doi.org/10.1002/jcb.29545 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....87c0ea22f3d8fcf49be56c448354e71a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974644 07302312 |
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| DOI: | 10.1002/jcb.29545 |