Biological Function and Mechanism of Long Noncoding RNAs Nuclear-Enriched Abundant Transcript 1 in Development of Cervical Cancer
| العنوان: | Biological Function and Mechanism of Long Noncoding RNAs Nuclear-Enriched Abundant Transcript 1 in Development of Cervical Cancer |
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| المؤلفون: | Hai-Bo Li, Shun-Yu Hou, Cai-Ping Mao, Le Bo, Hui-Ling Wang, Jianping Qiu |
| المصدر: | Chinese Medical Journal Chinese Medical Journal, Vol 131, Iss 17, Pp 2063-2070 (2018) |
| بيانات النشر: | Medknow Publications & Media Pvt Ltd, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Cell, Proliferation, lcsh:Medicine, Uterine Cervical Neoplasms, medicine.disease_cause, Cervical Cancer, HeLa, 03 medical and health sciences, Phosphatidylinositol 3-Kinases, 0302 clinical medicine, PI3K/Akt Signaling Pathway, Cell Line, Tumor, medicine, Humans, Migration, Cell Proliferation, Cervical cancer, Gene knockdown, biology, Akt/PKB signaling pathway, Cell growth, lcsh:R, Long Noncoding RNA Nuclear-Enriched Abundant Transcript 1, General Medicine, Middle Aged, medicine.disease, biology.organism_classification, 030104 developmental biology, medicine.anatomical_structure, Cell culture, 030220 oncology & carcinogenesis, Cancer research, Original Article, Female, RNA, Long Noncoding, Carcinogenesis, HeLa Cells |
| الوصف: | Background: Accumulating documents have demonstrated that long noncoding RNAs (lncRNAs) play critical roles in tumorigenesis. As an lncRNA, nuclear-enriched abundant transcript 1 (NEAT1) has been identified to be involved in the progression of many types of cancers. However, the biological function of NEAT1 in cervical cancer is not fully investigated. The aim of this study was to disclose the specific biological function of lncRNA NEAT1 in cervical cancer progression. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to identify the expression of lncRNA NEAT1 in the cervical cancer tissues and cell lines. All cervical cancer samples used in this study were collected from the Affiliated Suzhou Hospital of Nanjing Medical University between September 2012 and September 2017. The correlation between NEAT1 expression and the overall survival rate of cervical cancer patients was analyzed by Kaplan-Meier analysis. The effects of NEAT1 knockdown or overexpression on cell proliferation were tested by performing MTT assays and colony formation assays. Transwell assays were conducted to detect the migratory ability of cervical cancer cells, in which NEAT1 was silenced or overexpressed. Western blotting was utilized to validate whether NEAT1 promotes cervical cancer progression through activating PI3K-Akt signaling pathway. Results: High expression of NEAT1 predicted poor prognosis of cervical cancer patients (χ2 = 0.735, P = 0.005). Knockdown of NEAT1 decreased the number of colonies in CaSki cell from 136.667 ± 13.503 to 71.667 ± 7.506 (t= −18.76, P = 0.003) and decreased the number of colonies in HeLa cell from 128.667 ± 13.317 to 65.667 ± 7.024 (t = −5.54, P = 0.031). However, overexpression of NEAT1 increased the number of colonies in SiHa cell from 84.667 ± 12.014 to 150.667 ± 18.037 (t = 7.27, P = 0.018). Knockdown of NEAT1 decreased the migratory number of CaSki cell from 100.333 ± 9.866 to 58.333 ± 5.859 (t = −8.08, P = 0.015) and reduced the migratory number in HeLa cell from 123.667 ± 12.097 to 67.667 ± 7.095 (t = −6.03, P = 0.026). Overexpression of NEAT1 increased the migratory number of SiHa cell from 127.333 ± 16.042 to 231.333 ± 31.786 (t = 4.92, P = 0.039). Conclusion: NEAT1 may exert oncogenic function in cervical cancer and serve as a novel therapeutic target for cervical cancer. Key words: Cervical Cancer; Long Noncoding RNA Nuclear-Enriched Abundant Transcript 1; Migration; PI3K/Akt Signaling Pathway; Proliferation |
| اللغة: | English |
| تدمد: | 0366-6999 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::879056cf421a5a87ab932cb490782f65 http://europepmc.org/articles/PMC6111676 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....879056cf421a5a87ab932cb490782f65 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 03666999 |
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