Amnesiac Is Required in the Adult Mushroom Body for Memory Formation
| العنوان: | Amnesiac Is Required in the Adult Mushroom Body for Memory Formation |
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| المؤلفون: | Thomas Preat, Valérie Goguel, Oriane Turrel |
| المساهمون: | Laboratoire Gènes et Dynamique des Systèmes de Mémoire (GDSM), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Plasticité du Cerveau Brain Plasticity (UMR 8249) (PdC) |
| المصدر: | Journal of Neuroscience Journal of Neuroscience, Society for Neuroscience, 2018, 38 (43), pp.9202-9214. ⟨10.1523/JNEUROSCI.0876-18.2018⟩ |
| بيانات النشر: | Society for Neuroscience, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, endocrine system, [SDV]Life Sciences [q-bio], Neuropeptide, Biology, Animals, Genetically Modified, 03 medical and health sciences, Transcription (biology), RNA interference, Enhancer trap, Animals, Drosophila Proteins, Olfactory memory, Enhancer, Research Articles, Mushroom Bodies, Memory Consolidation, amnesiac, Neurons, dorsal paired medial neurons, General Neuroscience, Neuropeptides, Age Factors, mushroom body, 030104 developmental biology, Drosophila melanogaster, Mushroom bodies, Drosophila, Female, olfactory memory, Olfactory Learning, Neuroscience |
| الوصف: | It was proposed that theDrosophila amnesiacgene (amn) is required for consolidation of aversive memory in the dorsal paired medial (DPM) neurons, a pair of large neurons that broadly innervate the mushroom bodies (MB), the fly center for olfactory learning and memory (Waddell et al., 2000). Yet, a conditional analysis showed that it was not possible to rescue the memory deficit ofamnX8null mutant flies whenamnexpression was restored only in the adult (DeZazzo et al., 1999), which led the authors to suggest thatamnmight be involved in the development of brain structures that normally promote adult olfactory memory. To further investigate temporal and spatial requirements of Amnesiac (AMN) peptide in memory, we used RNA interference in combination with conditional drivers. Experiments were conducted either in both sexes, or in either sexes. Our data show that acute modulation ofamnexpression in adult DPM neurons does not impact memory. We further show thatamnexpression is required for normal development of DPM neurons. Detailed enhancer trap analyses suggest thatamntranscription unit contains two distinct enhancers, one specific of DPM neurons, and the other specific of α/β MB neurons. This prompted us to investigate extensively the role of AMN in the adult MB. Together, our results demonstrate thatamnis acutely required in adult α/β MB neurons for middle-term and long-term memory. The data thus establish thatamnplays two distinct roles. Its expression is required in DPM neurons for their development, and in adult MB for olfactory memory.SIGNIFICANCE STATEMENTTheDrosophila amnesiacgene encodes a neuropeptide whose expression was proposed to be required for consolidation of aversive memory in the dorsal paired medial (DPM) neurons, a pair of large neurons that broadly innervate the mushroom bodies (MB), the olfactory memory center. Here, we investigatedamnesiactemporal and spatial requirement using conditional tools that allowed us to manipulate its expression in selected neurons. This work leads to a complete reassessment of the role ofamnesiacin brain development and memory. We show thatamnesiacis required for two distinct processes: for normal development of DPM neurons, and in adult MB for memory. |
| اللغة: | English |
| تدمد: | 0270-6474 1529-2401 |
| DOI: | 10.1523/JNEUROSCI.0876-18.2018⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85ab605ddaf1e686de361afae87a4037 https://europepmc.org/articles/PMC6705992/ |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....85ab605ddaf1e686de361afae87a4037 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 02706474 15292401 |
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| DOI: | 10.1523/JNEUROSCI.0876-18.2018⟩ |