Transcriptomic analysis of trout gill ionocytes in fresh water and sea water using laser capture microdissection combined with microarray analysis
| العنوان: | Transcriptomic analysis of trout gill ionocytes in fresh water and sea water using laser capture microdissection combined with microarray analysis |
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| المؤلفون: | Jérôme Montfort, Isabelle Leguen, Aurélie Le Cam, Alain Fautrel, Sandrine Peron |
| المساهمون: | Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Plateforme Génomique Santé Biogenouest®, Institut Fédératif de Recherche - Génétique Fonctionnelle Agronomie et Santé (IFR 140 GFAS), Hépatotoxicité et xénobiotiques, Détoxication et réparation Tissulaire, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Foie, métabolismes et cancer, Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Foie, métabolismes et cancer, Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Foie, métabolismes et cancer, Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Jonchère, Laurent |
| المصدر: | PLoS ONE PLoS ONE, Public Library of Science, 2015, 10 (10), pp.1-22. ⟨10.1371/journal.pone.0139938⟩ Plos One (10:10), 1-22. (2015) PLoS ONE, Public Library of Science, 2015, 10 (10), pp.e0139938. ⟨10.1371/journal.pone.0139938⟩ PLoS ONE, 2015, 10 (10), pp.1-22. ⟨10.1371/journal.pone.0139938⟩ PLoS ONE, Vol 10, Iss 10, p e0139938 (2015) |
| بيانات النشر: | HAL CCSD, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Gill, [SDV]Life Sciences [q-bio], lcsh:Medicine, adaptation, Transcriptome, 0302 clinical medicine, poisson, immune system proteins, salmonids, lcsh:Science, Oligonucleotide Array Sequence Analysis, Laser capture microdissection, seawater, salmonidae, 0303 health sciences, trout, oncorhynchus mykiss, Multidisciplinary, gills, biology, homéostasie ionique, rainbow trout, 6. Clean water, Cell biology, [SDV] Life Sciences [q-bio], Trout, fresh water, protein metabolism, ionocyte, Research Article, expression des gènes, sea water, DNA-binding proteins, gene regulation, animal structures, Laser Capture Microdissection, 03 medical and health sciences, rainbow, Animals, 14. Life underwater, 030304 developmental biology, fish, Ion Transport, Microarray analysis techniques, Gene Expression Profiling, lcsh:R, gill, branchie, sweet water, biology.organism_classification, Molecular biology, Fold change, Gene expression profiling, Ion homeostasis, eau douce, eau de mer, Tissue Array Analysis, lcsh:Q, 030217 neurology & neurosurgery, truite arc en ciel |
| الوصف: | International audience; Fish gills represent a complex organ composed of several cell types that perform multiple physiological functions. Among these cells, ionocytes are implicated in the maintenance of ion homeostasis. However, because the ionocyte represents only a small percent of whole gill tissue, its specific transcriptome can be overlooked among the numerous cell types included in the gill. The objective of this study is to better understand ionocyte functions by comparing the RNA expression of this cell type in freshwater and seawater acclimated rainbow trout. To realize this objective, ionocytes were captured from gill cryosections using laser capture microdissection after immunohistochemistry. Then, transcriptome analyses were performed on an Agilent trout oligonucleotide microarray. Gene expression analysis identified 108 unique annotated genes differentially expressed between freshwater and seawater ionocytes, with a fold change higher than 3. Most of these genes were up-regulated in freshwater cells. Interestingly, several genes implicated in ion transport, extracellular matrix and structural cellular proteins appeared up-regulated in freshwater ionocytes. Among them, several ion transporters, such as CIC2, SLC26A6, and NBC, were validated by qPCR and/or in situ hybridization. The latter technique allowed us to localize the transcripts of these ion transporters in only ionocytes and more particularly in the freshwater cells. Genes involved in metabolism and also several genes implicated in transcriptional regulation, cell signaling and the cell cycle were also enhanced in freshwater ionocytes. In conclusion, laser capture microdissection combined with microarray analysis allowed for the determination of the transcriptional signature of scarce cells in fish gills, such as ionocytes, and aided characterization of the transcriptome of these cells in freshwater and seawater acclimated trout |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0139938⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84317bb9af5699576dc20ce37798b31d https://hal-univ-rennes1.archives-ouvertes.fr/hal-01216200/document |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....84317bb9af5699576dc20ce37798b31d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0139938⟩ |