DevT (Alr4674), resembling a Ser/Thr protein phosphatase, is essential for heterocyst function in the cyanobacterium Anabaena sp. PCC 7120
| العنوان: | DevT (Alr4674), resembling a Ser/Thr protein phosphatase, is essential for heterocyst function in the cyanobacterium Anabaena sp. PCC 7120 |
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| المؤلفون: | Javier Espinosa, Nicole Fiedler, Alicia M. Muro-Pastor, Karl Forchhammer, Thomas Brunner, Iris Maldener |
| المساهمون: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología, University of Tübingen. Interfaculty Institute for Microbiology and Infection Medicine, Organismic Interactions, University of Regensburg. Lehrstuhl fur Zellbiologie und Pflanzenphysiologie, Instituto de Bioquímica Vegetal y Fotosíntesis (CSIC-Universidad de Sevilla), Transducción de Señales en Bacterias |
| المصدر: | RUA. Repositorio Institucional de la Universidad de Alicante Universidad de Alicante (UA) Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | Microbiology Society, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Threonine, Cyanobacteria, biology, Anabaena, Cellular differentiation, Molecular Sequence Data, Mutant, Phosphatase, Nitrogenase, Gene Expression Regulation, Bacterial, biology.organism_classification, Genética, Microbiology, Heterocyst differentiation, Bacterial Proteins, Biochemistry, Phosphoprotein Phosphatases, Serine, Heterocyst |
| الوصف: | Heterocyst-forming cyanobacteria are able to perform oxygenic photosynthesis and nitrogen fixation simultaneously in the same filament, by restricting the highly O2-sensitive nitrogenase to specialized cells, the heterocysts. A remarkable change in morphology and metabolism accompanies the differentiation of heterocysts, which only occurs when no source of combined nitrogen is available. In this study, we characterized DevT (Alr4674), a putative protein phosphatase from Anabaena PCC 7120. Mutants defective in devT are able to form morphologically mature heterocysts, which however cannot fix N2, and the mutant cannot survive without a source of combined nitrogen. DevT shows homology to phosphatases of the PPP family and displays a Mn2+-dependent phosphatase activity that can be inhibited by phosphatase inhibitors and oxidizing conditions. DevT is constitutively expressed in both vegetative cells and heterocysts, and is not regulated by NtcA. The heterocyst regulator HetR may exert a certain inhibition on the expression of devT. Under diazotrophic growth conditions, DevT protein accumulates specifically in mature heterocysts. Therefore DevT plays a still unknown role in a late essential step of heterocyst differentiation. © 2010 SGM. |
| تدمد: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.043398-0 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::824d81cb0fa8383585510b1fa010f145 https://doi.org/10.1099/mic.0.043398-0 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....824d81cb0fa8383585510b1fa010f145 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652080 13500872 |
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| DOI: | 10.1099/mic.0.043398-0 |