Investigating the level of DNA double‐strand break in human spermatozoa and its relation to semen characteristics and IVF outcome using phospho‐histone H2AX antibody as a biomarker
| العنوان: | Investigating the level of DNA double‐strand break in human spermatozoa and its relation to semen characteristics and IVF outcome using phospho‐histone H2AX antibody as a biomarker |
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| المؤلفون: | Munevver Serdarogullari, Onder Coban, Nedime Serakinci, Zalihe Yarkiner |
| المصدر: | Andrology. 8:421-426 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Male, endocrine system, Urology, Endocrinology, Diabetes and Metabolism, Motility, Semen, Fertilization in Vitro, Male infertility, Histones, Andrology, Endocrinology, Pregnancy, medicine, Humans, DNA Breaks, Double-Stranded, Prospective Studies, Prospective cohort study, Infertility, Male, urogenital system, business.industry, Middle Aged, medicine.disease, Spermatozoa, Sperm, Reproductive Medicine, DNA fragmentation, Biomarker (medicine), Female, Live birth, business, Live Birth, Biomarkers |
| الوصف: | BACKGROUND Sperm DNA fragmentation and its relation to conventional semen parameters are well studied. However, there is limited information regarding the rate of DNA double-strand breaks (DSBs) and its correlation to basic semen parameters and IVF outcome. OBJECTIVES The present study aimed to investigate the rate of DNA DSBs in human spermatozoa and its correlation to basic semen parameters and IVF outcome. MATERIALS AND METHODS The prospective study includes 60 assisted reproductive treatment cycles (52 autologous and eight donors) in which the semen profiles and sperm DNA DSBs have been assessed. The level of sperm DNA DSBs in each sample has been evaluated by using a method to detect histone H2AX phosphorylation. The results were compared with basic semen values and IVF outcomes. RESULTS No significant correlation was observed between phospho-histone H2AX (γH2AX) levels and basic semen parameters such as semen volume (p = 0.129), sperm count (p = 0.454), total motility (p = 0.934), progressive motility (p = 0.314) and normal sperm morphology (p = 0.720). Similarly, the mean values of γH2AX did not differ with regard to the age of male participants (p = 0.300). However, cycles that resulted in live birth exhibited lower levels of γH2AX (p = 0.007). Accordingly, the level of γH2AX (p |
| تدمد: | 2047-2927 2047-2919 |
| DOI: | 10.1111/andr.12689 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80993a7970cc4ea0927431577a74970e https://doi.org/10.1111/andr.12689 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....80993a7970cc4ea0927431577a74970e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20472927 20472919 |
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| DOI: | 10.1111/andr.12689 |