The Oviductal Extracellular Vesicles’ RNA Cargo Regulates the Bovine Embryonic Transcriptome
| العنوان: | The Oviductal Extracellular Vesicles’ RNA Cargo Regulates the Bovine Embryonic Transcriptome |
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| المؤلفون: | Stefan Bauersachs, Pascal Mermillod, Carmen Almiñana |
| المساهمون: | Genetics and Functional Genomics Group, Universität Zürich [Zürich] = University of Zurich (UZH), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), European Project: 677353,H2020,H2020-SFS-2015-2,IMAGE(2016), University of Zurich, Almiñana, Carmen, Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| المصدر: | International Journal of Molecular Sciences, Vol 21, Iss 4, p 1303 (2020) International Journal of Molecular Sciences Volume 21 Issue 4 International Journal of Molecular Sciences, MDPI, 2020, 21 (4), pp.1-31. ⟨10.3390/ijms21041303⟩ International Journal of Molecular Sciences, 2020, 21 (4), pp.1-31. ⟨10.3390/ijms21041303⟩ |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, 0301 basic medicine, oviduct, 10077 Institute of Veterinary Anatomy, 1607 Spectroscopy, Oviducts, Transcriptome, lcsh:Chemistry, Freezing, Gene expression, Cluster Analysis, lcsh:QH301-705.5, Spectroscopy, 630 Agriculture, Gene Expression Regulation, Developmental, Embryo, General Medicine, 3. Good health, Computer Science Applications, Cell biology, embryonic structures, Female, 1606 Physical and Theoretical Chemistry, extracellular vesicles, endocrine system, animal structures, 1503 Catalysis, Embryonic Development, embryo, exosomes, Biology, Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, microRNA, 1312 Molecular Biology, 1706 Computer Science Applications, Animals, RNA, Messenger, ev-derived mrnas, Physical and Theoretical Chemistry, Molecular Biology, Messenger RNA, 030102 biochemistry & molecular biology, Sequence Analysis, RNA, 1604 Inorganic Chemistry, urogenital system, Organic Chemistry, Embryogenesis, RNA, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, Embryo, Mammalian, Embryonic stem cell, ev rna cargo, 10187 Department of Farm Animals, MicroRNAs, 030104 developmental biology, ev-derived mirnas, lcsh:Biology (General), lcsh:QD1-999, gene expression, 570 Life sciences, biology, Cattle, human activities, 1605 Organic Chemistry |
| الوصف: | Oviductal extracellular vesicles (oEVs) are emerging as key players in the gamete/embryo&ndash oviduct interactions that contribute to successful pregnancy. Various positive effects of oEVs on gametes and early embryos have been found in vitro. To determine whether these effects are associated with changes of embryonic gene expression, the transcriptomes of embryos supplemented with bovine fresh (FeEVs) or frozen (FoEVs) oEVs during in vitro culture compared to controls without oEVs were analyzed by low-input RNA sequencing. Analysis of RNA-seq data revealed 221 differentially expressed genes (DEGs) between FoEV treatment and control, 67 DEGs for FeEV and FoEV treatments, and minor differences between FeEV treatment and control (28 DEGs). An integrative analysis of mRNAs and miRNAs contained in oEVs obtained in a previous study with embryonic mRNA alterations pointed to direct effects of oEV cargo on embryos (1) by increasing the concentration of delivered transcripts (2) by translating delivered mRNAs to proteins that regulate embryonic gene expression and (3) by oEV-derived miRNAs which downregulate embryonic mRNAs or modify gene expression in other ways. Our study provided the first high-throughput analysis of the embryonic transcriptome regulated by oEVs, increasing our knowledge on the impact of oEVs on the embryo and revealing the oEV RNA components that potentially regulate embryonic development. |
| وصف الملف: | application/pdf; Bauersachs_et_al_ijms-21-01303.pdf - application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| DOI: | 10.3390/ijms21041303⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f8b1f007618581c3b251b9c513012ba https://www.mdpi.com/1422-0067/21/4/1303 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7f8b1f007618581c3b251b9c513012ba |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms21041303⟩ |