Development, Validation, and Interlaboratory Evaluation of a Quantitative Multiplexing Method To Assess Levels of Ten Endogenous Allergens in Soybean Seed and Its Application to Field Trials Spanning Three Growing Seasons
| العنوان: | Development, Validation, and Interlaboratory Evaluation of a Quantitative Multiplexing Method To Assess Levels of Ten Endogenous Allergens in Soybean Seed and Its Application to Field Trials Spanning Three Growing Seasons |
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| المؤلفون: | Ryan Christopher Hill, Rowel Tobias, Austin Xu, Jason Bradshaw, Guomin Shan, Barry W. Schafer, Trent J. Oman, Bhaskar Malayappan, Li Sheng, Rod A. Herman, Xiujuan Wang, Jeff Shippar |
| المصدر: | Journal of Agricultural and Food Chemistry. 65:5531-5544 |
| بيانات النشر: | American Chemical Society (ACS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Growing season, Natural variation, medicine.disease_cause, Mass Spectrometry, 0404 agricultural biotechnology, Allergen, Lc ms ms, medicine, Multiplex, Chromatography, High Pressure Liquid, Chemistry, business.industry, Genetically engineered, Soybean allergen, food and beverages, 04 agricultural and veterinary sciences, General Chemistry, Allergens, Antigens, Plant, Plants, Genetically Modified, 040401 food science, Biotechnology, Seeds, Soybean Proteins, Seasons, Soybeans, General Agricultural and Biological Sciences, business |
| الوصف: | As part of the regulatory approval process in Europe, comparison of endogenous soybean allergen levels between genetically engineered (GE) and non-GE plants has been requested. A quantitative multiplex analytical method using tandem mass spectrometry was developed and validated to measure 10 potential soybean allergens from soybean seed. The analytical method was implemented at six laboratories to demonstrate the robustness of the method and further applied to three soybean field studies across multiple growing seasons (including 21 non-GE soybean varieties) to assess the natural variation of allergen levels. The results show environmental factors contribute more than genetic factors to the large variation in allergen abundance (2- to 50-fold between environmental replicates) as well as a large contribution of Gly m 5 and Gly m 6 to the total allergen profile, calling into question the scientific rational for measurement of endogenous allergen levels between GE and non-GE varieties in the safety assessment. |
| تدمد: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.7b01018 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e7d00ae49f1d86e0b5fd3297c2ed1b8 https://doi.org/10.1021/acs.jafc.7b01018 |
| رقم الانضمام: | edsair.doi.dedup.....7e7d00ae49f1d86e0b5fd3297c2ed1b8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205118 00218561 |
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| DOI: | 10.1021/acs.jafc.7b01018 |