Caenorhabditis elegans algn-2 Is Critical for Longevity Conferred by Enhanced Nonsense-Mediated mRNA Decay
| العنوان: | Caenorhabditis elegans algn-2 Is Critical for Longevity Conferred by Enhanced Nonsense-Mediated mRNA Decay |
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| المؤلفون: | Yoonji Jung, Hae-Eun H. Park, Eun Ji Kim, Heehwa G. Son, Sujeong Kwon, Seung-Jae Lee |
| المصدر: | iScience |
| بيانات النشر: | Elsevier BV, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Messenger RNA, Multidisciplinary, media_common.quotation_subject, Nonsense-mediated decay, Longevity, RNA, Cell Biology, 02 engineering and technology, Biology, 021001 nanoscience & nanotechnology, biology.organism_classification, Article, Cell biology, 03 medical and health sciences, 030104 developmental biology, Downregulation and upregulation, Genetics, 0210 nano-technology, Molecular Biology, Transcription factor, Caenorhabditis elegans, media_common, Genetic screen |
| الوصف: | Summary Nonsense-mediated mRNA decay (NMD) is a biological surveillance mechanism that eliminates mRNA transcripts with premature termination codons. In Caenorhabditis elegans, NMD contributes to longevity by enhancing RNA quality. Here, we aimed at identifying NMD-modulating factors that are crucial for longevity in C. elegans by performing genetic screens. We showed that knocking down each of algn-2/asparagine-linked glycosylation protein, zip-1/bZIP transcription factor, and C44B11.1/FAS apoptotic inhibitory molecule increased the transcript levels of NMD targets. Among these, algn-2 exhibited an age-dependent decrease in its expression and was required for maintaining normal lifespan and for longevity caused by various genetic interventions. We further demonstrated that upregulation of ALGN-2 by inhibition of daf-2/insulin/IGF-1 receptor contributed to longevity in an NMD-dependent manner. Thus, algn-2, a positive regulator of NMD, plays a crucial role in longevity in C. elegans, likely by enhancing RNA surveillance. Our study will help understand how NMD-mediated mRNA quality control extends animal lifespan. Graphical Abstract Highlights • C. elegans algn-2 is a positive regulator of nonsense-mediated mRNA decay (NMD) • algn-2 is downregulated during aging and contributes to maintaining normal lifespan • algn-2 is required for longevity caused by various genetic interventions • Upregulation of ALGN-2 by inhibition of daf-2 promotes longevity via increasing NMD Genetics; Molecular Biology; Cell Biology |
| تدمد: | 2589-0042 |
| DOI: | 10.1016/j.isci.2020.101713 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e12c795831a08b2c73cd6af1a0680f8 https://doi.org/10.1016/j.isci.2020.101713 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7e12c795831a08b2c73cd6af1a0680f8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 25890042 |
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| DOI: | 10.1016/j.isci.2020.101713 |