التفاصيل البيبلوغرافية
| العنوان: |
Excess Centrosomes Disrupt Vascular Lumenization and Endothelial Cell Adherens Junctions |
| المؤلفون: |
Danielle B Buglak, Erich J Kushner, Allison P Marvin, Katy L Davis, Victoria L Bautch |
| بيانات النشر: |
Cold Spring Harbor Laboratory, 2019. |
| سنة النشر: |
2019 |
| مصطلحات موضوعية: |
Sprouting angiogenesis, 0303 health sciences, biology, Chemistry, Microtubule organizing center, biology.organism_classification, Cell biology, Adherens junction, Endothelial stem cell, 03 medical and health sciences, 0302 clinical medicine, medicine.anatomical_structure, Centrosome, 030220 oncology & carcinogenesis, Cell polarity, medicine, Zebrafish, 030304 developmental biology, Blood vessel |
| الوصف: |
Proper blood vessel formation requires coordinated changes in endothelial cell polarity and rearrangement of cell-cell junctions to form a functional lumen. One important regulator of cell polarity is the centrosome, which acts as a microtubule organizing center. Excess centrosomes perturb aspects of endothelial cell polarity linked to migration, but whether centrosome number influences apical-basal polarity and cell-cell junctions is unknown. Here, we show that excess centrosomes alter the apical-basal polarity of endothelial cells in angiogenic sprouts and disrupt endothelial cell-cell adherens junctions. Endothelial cells with excess centrosomes had narrower lumens in a 3D sprouting angiogenesis model, and zebrafish intersegmental vessels had reduced perfusion following centrosome overduplication. These results indicate that endothelial cell centrosome number regulates proper lumenization downstream of effects on apical-basal polarity and cell-cell junctions. Endothelial cells with excess centrosomes are prevalent in tumor vessels, suggesting how centrosomes may contribute to tumor vessel dysfunction. |
| اللغة: |
English |
| DOI: |
10.1101/863787 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d27e2fc936d52b2689f2a16bc49463a |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....7d27e2fc936d52b2689f2a16bc49463a |
| قاعدة البيانات: |
OpenAIRE |