An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming
| العنوان: | An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming |
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| المؤلفون: | Ferraz, Marcia A M M, Rho, Hoon Suk, Hemerich, Daiane, Henning, Heiko H W, van Tol, Helena T A, Hölker, Michael, Besenfelder, Urban, Mokry, Michal, Vos, Peter L A M, Stout, Tom A E, Le Gac, Séverine, Gadella, Bart M, LS Voortplanting Inwendige Ziekten, dES/dFAH FR, Sub Celbiologisch lab., dFAH AVR, LS Klinische Reproductie, Sub Biologie van de mannelijke gameet, Sub Reproductie mannelijk, dB&C FR-RMSC FR |
| المساهمون: | LS Voortplanting Inwendige Ziekten, dES/dFAH FR, Sub Celbiologisch lab., dFAH AVR, LS Klinische Reproductie, Sub Biologie van de mannelijke gameet, Sub Reproductie mannelijk, dB&C FR-RMSC FR |
| المصدر: | Nature Communications Nature Communications, Vol 9, Iss 1, Pp 1-14 (2018) Nature Communications, 9(1). Nature Publishing Group Nature communications, 9:4934. Nature Publishing Group |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Zygote, medicine.medical_treatment, General Physics and Astronomy, Oviducts, Cellular Reprogramming/genetics, Epigenesis, Genetic, Human fertilization, Pregnancy, Zygote/growth & development, Non-U.S. Gov't, lcsh:Science, Cells, Cultured, Cultured, Multidisciplinary, Research Support, Non-U.S. Gov't, Embryo, Genomics, Cellular Reprogramming, 3. Good health, Cell biology, Fertilization in Vitro/instrumentation, Oviduct, Oviducts/cytology, Female, Reprogramming, Science, Cells, Fertilization in Vitro, Biology, Research Support, Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Genetic, Journal Article, medicine, Animals, Humans, Assisted reproductive technology, Gene Expression Profiling, Embryo culture, General Chemistry, Embryonic stem cell, Genomics/methods, Gene Ontology, 030104 developmental biology, lcsh:Q, Cattle, Epigenesis |
| الوصف: | Worldwide over 5 million children have been conceived using assisted reproductive technology, and research has concentrated on increasing the likelihood of ongoing pregnancy. However, studies using animal models have indicated undesirable effects of in vitro embryo culture on offspring development and health. In vivo, the oviduct hosts a period in which the early embryo undergoes complete reprogramming of its (epi)genome in preparation for the reacquisition of (epi)genetic marks. We designed an oviduct-on-a-chip platform to better investigate the mechanisms related to (epi)genetic reprogramming and the degree to which they differ between in vitro and in vivo embryos. The device supports more physiological (in vivo-like) zygote genetic reprogramming than conventional IVF. This approach will be instrumental in identifying and investigating factors critical to fertilization and pre-implantation development, which could improve the quality and (epi)genetic integrity of IVF zygotes with likely relevance for early embryonic and later fetal development. In vitro culture has detrimental effects on transcriptomes and epigenetic programming of zygotes. Here the authors use microfluidic technology to co-culture bovine oviduct epithelial cells with zygotes and show that the transcriptomes and global methylation patterns of these zygotes are more similar to in vivo zygotes than to conventionally cultured zygotes. |
| وصف الملف: | image/pdf; application/pdf |
| تدمد: | 2041-1723 |
| DOI: | 10.1038/s41467-018-07119-8 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d2541d45c82fec9aa3455a5d459a09e https://doi.org/10.1038/s41467-018-07119-8 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7d2541d45c82fec9aa3455a5d459a09e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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| DOI: | 10.1038/s41467-018-07119-8 |