SARS-CoV-2 Infected Pediatric Cerebral Cortical Neurons: Transcriptomic Analysis and Potential Role of Toll-like Receptors in Pathogenesis
| العنوان: | SARS-CoV-2 Infected Pediatric Cerebral Cortical Neurons: Transcriptomic Analysis and Potential Role of Toll-like Receptors in Pathogenesis |
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| المؤلفون: | Mara Biasin, Valeria Calcaterra, Gioia Cappelletti, Placido Bramanti, Gian Vincenzo Zuccotti, Gloria Pelizzo, Emanuela Mazzon, Luigi Chiricosta, Stephana Carelli, Agnese Gugliandolo, Maria Antonietta Avanzini |
| المصدر: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 8059, p 8059 (2021) Volume 22 Issue 15 |
| بيانات النشر: | MDPI, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Chemokine, QH301-705.5, viruses, Biology, Virus Replication, Catalysis, Article, Inorganic Chemistry, Transcriptome, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, children, Humans, Biology (General), Physical and Theoretical Chemistry, Scavenger receptor, Receptor, Child, QD1-999, Molecular Biology, Spectroscopy, transcriptomic analysis, Regulation of gene expression, Cerebral Cortex, Neurons, SARS-CoV-2, Gene Expression Profiling, Organic Chemistry, nervous system, Toll-Like Receptors, Pattern recognition receptor, COVID-19, General Medicine, human neuronal cells, Computer Science Applications, Cell biology, Chemistry, 030104 developmental biology, Gene Expression Regulation, biology.protein, Cytokines, Signal transduction, 030217 neurology & neurosurgery, Signal Transduction |
| الوصف: | Different mechanisms were proposed as responsible for COVID-19 neurological symptoms but a clear one has not been established yet. In this work we aimed to study SARS-CoV-2 capacity to infect pediatric human cortical neuronal HCN-2 cells, studying the changes in the transcriptomic profile by next generation sequencing. SARS-CoV-2 was able to replicate in HCN-2 cells, that did not express ACE2, confirmed also with Western blot, and TMPRSS2. Looking for pattern recognition receptor expression, we found the deregulation of scavenger receptors, such as SR-B1, and the downregulation of genes encoding for Nod-like receptors. On the other hand, TLR1, TLR4 and TLR6 encoding for Toll-like receptors (TLRs) were upregulated. We also found the upregulation of genes encoding for ERK, JNK, NF-κB and Caspase 8 in our transcriptomic analysis. Regarding the expression of known receptors for viral RNA, only RIG-1 showed an increased expression downstream RIG-1, the genes encoding for TRAF3, IKKε and IRF3 were downregulated. We also found the upregulation of genes encoding for chemokines and accordingly we found an increase in cytokine/chemokine levels in the medium. According to our results, it is possible to speculate that additionally to ACE2 and TMPRSS2, also other receptors may interact with SARS-CoV-2 proteins and mediate its entry or pathogenesis in pediatric cortical neurons infected with SARS-CoV-2. In particular, TLRs signaling could be crucial for the neurological involvement related to SARS-CoV-2 infection. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c5e4b1608a60db4aa08fa7fc47330c4 http://europepmc.org/articles/PMC8347089 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7c5e4b1608a60db4aa08fa7fc47330c4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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