Meticulous Plasma Isolation is Essential to Avoid False Low-Level Viraemia in Roche Cobas HIV-1 Viral Load Assays
| العنوان: | Meticulous Plasma Isolation is Essential to Avoid False Low-Level Viraemia in Roche Cobas HIV-1 Viral Load Assays |
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| المؤلفون: | Sylvie Dinakis, Jean Ruelle, Els Demecheleer, Chris Verhofstede, Tom Van Maerken, Delfien Staelens, Géraldine Dessilly, Marlies Schauvliege, Kenny Dauwe, Virginie Mortier, Leen Vancoillie |
| المصدر: | Antiviral Therapy. 23:277-281 |
| بيانات النشر: | SAGE Publications, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Isolation (health care), 030106 microbiology, Sample processing, Human immunodeficiency virus (HIV), HIV Infections, Real-Time Polymerase Chain Reaction, medicine.disease_cause, Sensitivity and Specificity, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Humans, Medicine, Pharmacology (medical), Viremia, 030212 general & internal medicine, Pharmacology, business.industry, RNA, DNA Contamination, Viral Load, Virology, Infectious Diseases, chemistry, HIV-1, RNA, Viral, Biological Assay, Reagent Kits, Diagnostic, business, Viral load, DNA |
| الوصف: | Background Pre-analytical sample processing is often overlooked as a potential cause of inaccurate assay results. Here we demonstrate how plasma, extracted from standard EDTA-containing blood collection tubes, may contain traces of blood cells consequently resulting in a false low-level HIV-1 viral load when using Roche Cobas HIV-1 assays. Methods The presence of human DNA in Roche Cobas 4800 RNA extracts and in RNA extracts from the Abbott HIV-1 RealTime assay was assessed by quantifying the human albumin gene by means of quantitative PCR. RNA was extracted from plasma samples before and after an additional centrifugation and tested for viral load and DNA contamination. The relation between total DNA content and viral load was defined. Results Elevated concentrations of genomic DNA were detected in 28 out of 100 Cobas 4800 extracts and were significantly more frequent in samples processed outside of the AIDS Reference Laboratory. An association between genomic DNA presence and spurious low-level viraemia results was demonstrated. Supplementary centrifugation of plasma before RNA extraction eliminated the contamination and the false viraemia. Conclusions Plasma isolated from standard EDTA-containing blood collection tubes may contain traces of HIV DNA leading to false viral load results above the clinical cutoff. Supplementary centrifugation of plasma before viral load analysis may eliminate the occurrence of this spurious low-level viraemia. |
| تدمد: | 2040-2058 1359-6535 |
| DOI: | 10.3851/imp3203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c49e16cb7c87e1289d7cbc2e4216830 https://doi.org/10.3851/imp3203 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....7c49e16cb7c87e1289d7cbc2e4216830 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20402058 13596535 |
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| DOI: | 10.3851/imp3203 |