Regulation of osteogenesis via miR-101-3p in mesenchymal stem cells by human gingival fibroblasts
| العنوان: | Regulation of osteogenesis via miR-101-3p in mesenchymal stem cells by human gingival fibroblasts |
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| المؤلفون: | Mikihito Kajiya, Hiroyuki Kawaguchi, Tomoyuki Iwata, Takayoshi Nagahara, Eri Kaneda-Ikeda, Kazuhisa Ouhara, Shu Ishida, Minami Yoshioka, Hidemi Kurihara, Noriyoshi Mizuno |
| المصدر: | Journal of Bone and Mineral Metabolism. 38:442-455 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Gingiva, 030209 endocrinology & metabolism, Biology, Regenerative medicine, ETV1, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Osteogenesis, microRNA, medicine, Humans, Orthopedics and Sports Medicine, Secretion, Insulin-Like Growth Factor I, Transcription factor, Cells, Cultured, Growth factor, Regeneration (biology), Mesenchymal stem cell, Cell Differentiation, Mesenchymal Stem Cells, General Medicine, Fibroblasts, Cell biology, MicroRNAs, 030101 anatomy & morphology, Biomarkers, Transcription Factors |
| الوصف: | Mesenchymal stem cells (MSCs) can differentiate into various types of cells and can thus be used for periodontal regenerative therapy. However, the mechanism of differentiation is still unclear. Transplanted MSCs are, via their transcription factors or microRNAs (miRNAs), affected by periodontal cells with direct contact or secretion of humoral factors. Therefore, transplanted MSCs are regulated by humoral factors from human gingival fibroblasts (HGF). Moreover, insulin-like growth factor (IGF)-1 is secreted from HGF and regulates periodontal regeneration. To clarify the regulatory mechanism for MSC differentiation by humoral factors from HGF, we identified key genes, specifically miRNAs, involved in this process, and determined their function in MSC differentiation. Mesenchymal stem cells were indirectly co-cultured with HGF in osteogenic or growth conditions and then evaluated for osteogenesis, undifferentiated MSC markers, and characteristic miRNAs. MSCs had their miRNA expression levels adjusted or were challenged with IGF-1 during osteogenesis, or both of which were performed, and then, MSCs were evaluated for osteogenesis or undifferentiated MSC markers. Mesenchymal stem cells co-cultured with HGF showed suppression of osteogenesis and characteristic expression of ETV1, an undifferentiated MSC marker, as well as miR-101-3p. Over-expression of miR-101-3p regulated osteogenesis and ETV1 expression as well as indirect co-culture with HGF. IGF-1 induced miR-101-3p and ETV1 expression. However, IGF-1 did not suppress osteogenesis. Humoral factors from HGF suppressed osteogenesis in MSCs. The effect was regulated by miRNAs and undifferentiated MSC markers. miR-101-3p and ETV1 were the key factors and were regulated by IGF-1. |
| تدمد: | 1435-5604 0914-8779 |
| DOI: | 10.1007/s00774-019-01080-2 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c39aac67cae49b152e5c8b5c8fdd041 https://doi.org/10.1007/s00774-019-01080-2 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....7c39aac67cae49b152e5c8b5c8fdd041 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14355604 09148779 |
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| DOI: | 10.1007/s00774-019-01080-2 |