التفاصيل البيبلوغرافية
| العنوان: |
Association of VPg and eIF4E in the host tropism at the cellular level of Barley yellow mosaic virus and Wheat yellow mosaic virus in the genus Bymovirus |
| المؤلفون: |
Huangai Li, Yukio Shirako |
| المصدر: |
Virology. 476:159-167 |
| بيانات النشر: |
Elsevier BV, 2015. |
| سنة النشر: |
2015 |
| مصطلحات موضوعية: |
Barley yellow mosaic virus (BaYMV), Host tropism, Replication, Virus Replication, Genome, Viral genome-linked protein (VPg), Host Specificity, Viral Proteins, Barley yellow mosaic virus, Mesophyll protoplasts, Virology, Eukaryotic translation initiation factor 4E (eIF4E), Gene, Triticum, Host factor, Plant Diseases, Plant Proteins, Genetics, biology, Host (biology), fungi, RNA, food and beverages, Hordeum, Potyviridae, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Viral Tropism, Eukaryotic Initiation Factor-4E, Wheat yellow mosaic virus (WYMV), Wheat yellow mosaic virus, Bymovirus, Protein Binding |
| الوصف: |
Barley yellow mosaic virus (BaYMV) and Wheat yellow mosaic virus (WYMV) are separate species in the genus Bymovirus with bipartite plus-sense RNA genomes. In fields, BaYMV infects only barley and WYMV infects only wheat. Here, we studied the replicative capability of the two viruses in barley and wheat mesophyll protoplasts. BaYMV replicated in both barley and wheat protoplasts, but WYMV replicated only in wheat protoplasts. The expression of wheat translation initiation factor 4E (eIF4E), a common host factor for potyviruses, from the WYMV genome enabled WYMV replication in barley protoplasts. Replacing the BaYMV VPg gene with that of WYMV abolished BaYMV replication in barley protoplasts, whereas the additional expression of wheat eIF4E from BaYMV genome restored the replication of the BaYMV mutant in barley protoplasts. These results indicate that both VPg and the host eIF4E are involved in the host tropism of BaYMV and WYMV at the replication level. |
| تدمد: |
0042-6822 |
| DOI: |
10.1016/j.virol.2014.12.010 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7abc7fab4d5933b81675a2f89c2a3bb6 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....7abc7fab4d5933b81675a2f89c2a3bb6 |
| قاعدة البيانات: |
OpenAIRE |