An Intact Cell Bioluminescence-Based Assay for the Simple and Rapid Diagnosis of Urinary Tract Infection
| العنوان: | An Intact Cell Bioluminescence-Based Assay for the Simple and Rapid Diagnosis of Urinary Tract Infection |
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| المؤلفون: | David Broyles, Edward Quinto, Jonathan Upegui, Sherwin Reyes, Sylvia Daunert, Sapna K. Deo, Emre Dikici, Mary Denneth Fuentes, Nga Le |
| المصدر: | International Journal of Molecular Sciences, Vol 21, Iss 5015, p 5015 (2020) International Journal of Molecular Sciences Volume 21 Issue 14 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Luminescence, Time Factors, Biosensing Techniques, Urine, medicine.disease_cause, urologic and male genital diseases, lcsh:Chemistry, Limit of Detection, Candida albicans, Medicine, Pathogen, lcsh:QH301-705.5, Spectroscopy, biology, Candidiasis, Bacterial Infections, General Medicine, bioluminescence, female genital diseases and pregnancy complications, Computer Science Applications, Staphylococcus aureus, Urinary Tract Infections, Urinary system, 030106 microbiology, biosensor, Article, Catalysis, Microbiology, Inorganic Chemistry, 03 medical and health sciences, Antibiotic resistance, Escherichia coli, Humans, Physical and Theoretical Chemistry, Proteus mirabilis, Molecular Biology, Photobacterium, business.industry, fungi, Organic Chemistry, biology.organism_classification, urinary tract infection diagnosis, 030104 developmental biology, rapid detection, lcsh:Biology (General), lcsh:QD1-999, Luminescent Measurements, business |
| الوصف: | Urinary tract infection (UTI) is one of the most common infections, accounting for a substantial portion of outpatient hospital and clinic visits. Standard diagnosis of UTI by culture and sensitivity can take at least 48 h, and improper diagnosis can lead to an increase in antibiotic resistance following therapy. To address these shortcomings, rapid bioluminescence assays were developed and evaluated for the detection of UTI using intact, viable cells of Photobacterium mandapamensis USTCMS 1132 or previously lyophilized cells of Photobacterium leiognathi ATCC 33981&trade Two platform technologies&mdash tube bioluminescence extinction technology urine (TuBETUr) and cellphone-based UTI bioluminescence extinction technology (CUBET)&mdash were developed and standardized using artificial urine to detect four commonly isolated UTI pathogens&mdash namely, Escherichia coli, Proteus mirabilis, Staphylococcus aureus, and Candida albicans. Besides detection, these assays could also provide information regarding pathogen concentration/level, helping guide treatment decisions. These technologies were able to detect microbes associated with UTI at less than 105 CFU/mL, which is usually the lower cut-off limit for a positive UTI diagnosis. Among the 29 positive UTI samples yielding 105&ndash 106 CFU/mL pathogen concentrations, a total of 29 urine specimens were correctly detected by TuBETUr as UTI-positive based on an 1119 s detection window. Similarly, the rapid CUBET method was able to discriminate UTIs from normal samples with high confidence (p &le 0.0001), using single-pot conditions and cell phone-based monitoring. These technologies could potentially address the need for point-of-care UTI detection while reducing the possibility of antibiotic resistance associated with misdiagnosed cases of urinary tract infections, especially in low-resource environments. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7932d8e60188243e363d4c8199f229a3 https://www.mdpi.com/1422-0067/21/14/5015 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7932d8e60188243e363d4c8199f229a3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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