m-AAA Complexes Are Not Crucial for the Survival of Arabidopsis Under Optimal Growth Conditions Despite Their Importance for Mitochondrial Translation
| العنوان: | m-AAA Complexes Are Not Crucial for the Survival of Arabidopsis Under Optimal Growth Conditions Despite Their Importance for Mitochondrial Translation |
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| المؤلفون: | Marta Kolodziejczak, Hanna Janska, Renata Skibior-Blaszczyk |
| المصدر: | Plant and Cell Physiology. 59:1006-1016 |
| بيانات النشر: | Oxford University Press (OUP), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | DNA, Bacterial, 0301 basic medicine, Proteases, Physiology, Mitochondrial translation, Mutant, Arabidopsis, Plant Development, Saccharomyces cerevisiae, Plant Science, Oxidative phosphorylation, Mitochondrion, Oxidative Phosphorylation, 03 medical and health sciences, Gene Expression Regulation, Plant, Amino Acid Sequence, RNA, Messenger, Regulation of gene expression, biology, Arabidopsis Proteins, Metalloendopeptidases, Cell Biology, General Medicine, biology.organism_classification, Mitochondria, Cell biology, Protein Subunits, 030104 developmental biology, Protein Biosynthesis, Mutation, Proteolysis, Protein Processing, Post-Translational, Ribosomes, Biogenesis, Molecular Chaperones |
| الوصف: | For optimal mitochondrial activity, the mitochondrial proteome must be properly maintained or altered in response to developmental and environmental stimuli. Based on studies of yeast and humans, one of the key players in this control are m-AAA proteases, mitochondrial inner membrane-bound ATP-dependent metalloenzymes. This study focuses on the importance of m-AAA proteases in plant mitochondria, providing their first experimentally proven physiological substrate. We found that the Arabidopsis m- AAA complexes composed of AtFTSH3 and/or AtFTSH10 are involved in the proteolytic maturation of ribosomal subunit L32. Consequently, in the double Arabidopsis ftsh3/10 mutant, mitoribosome biogenesis, mitochondrial translation and functionality of OXPHOS (oxidative phosphorylation) complexes are impaired. However, in contrast to their mammalian or yeast counterparts, plant m-AAA complexes are not critical for the survival of Arabidopsis under optimal conditions; ftsh3/10 plants are only slightly smaller in size at the early developmental stage compared with plants containing m-AAA complexes. Our data suggest that a lack of significant visible morphological alterations under optimal growth conditions involves mechanisms which rely on existing functional redundancy and induced functional compensation in Arabidopsis mitochondria. |
| تدمد: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcy041 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78871c3c11f9435f079862a6b3d07d5f https://doi.org/10.1093/pcp/pcy041 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....78871c3c11f9435f079862a6b3d07d5f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14719053 00320781 |
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| DOI: | 10.1093/pcp/pcy041 |