The role of P2Y12 in the kinetics of microglial self-renewal and maturation in the adult visual cortex in vivo
| العنوان: | The role of P2Y12 in the kinetics of microglial self-renewal and maturation in the adult visual cortex in vivo |
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| المؤلفون: | Antonio Ladron-de-Guevara, Jason Atlas, Cassandra E. Lamantia, Zachary Brehm, Monique S Mendes, Ania K. Majewska, Matthew N. McCall, Evelyn Matei, Linh Le |
| المصدر: | eLife eLife, Vol 10 (2021) |
| بيانات النشر: | eLife Sciences Publications, Ltd, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, in vivo two photon imaging, repopulation, Mouse, QH301-705.5, Science, microglia, Biology, General Biochemistry, Genetics and Molecular Biology, Colony stimulating factor 1 receptor, 03 medical and health sciences, Mice, 0302 clinical medicine, Immune system, P2Y12, In vivo, Cell Movement, medicine, Animals, Biology (General), Cell Self Renewal, Receptor, Visual Cortex, Mice, Knockout, General Immunology and Microbiology, Microglia, General Neuroscience, Brain, General Medicine, Models, Theoretical, Receptors, Purinergic P2Y12, Mice, Inbred C57BL, PLX5622, Kinetics, 030104 developmental biology, Visual cortex, medicine.anatomical_structure, nervous system, ontogeny, Knockout mouse, Medicine, Neuroscience, 030217 neurology & neurosurgery, Research Article, colony stimulating factor 1 receptor, Signal Transduction |
| الوصف: | Microglia are the brain’s resident immune cells with a tremendous capacity to autonomously self-renew. Because microglial self-renewal has largely been studied using static tools, its mechanisms and kinetics are not well understood. Using chronic in vivo two-photon imaging in awake mice, we confirm that cortical microglia show limited turnover and migration under basal conditions. Following depletion, however, microglial repopulation is remarkably rapid and is sustained by the dynamic division of remaining microglia, in a manner that is largely independent of signaling through the P2Y12 receptor. Mathematical modeling of microglial division demonstrates that the observed division rates can account for the rapid repopulation observed in vivo. Additionally, newly born microglia resemble mature microglia within days of repopulation, although morphological maturation is different in newly born microglia in P2Y12 knock out mice. Our work suggests that microglia rapidly locally and that newly born microglia do not recapitulate the slow maturation seen in development but instead take on mature roles in the CNS. |
| اللغة: | English |
| تدمد: | 2050-084X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::785abce2459e5c14190c6030f76e3627 http://europepmc.org/articles/PMC8341987 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....785abce2459e5c14190c6030f76e3627 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2050084X |
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