Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host–Pathogen Response to Infection
| العنوان: | Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host–Pathogen Response to Infection |
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| المؤلفون: | Vianey Paola Barrera Enriquez, Dany Beste, Luis Barrera, Víctor A. López-Agudelo, FELIPE CABARCAS JARAMILLO, Andres Baena, Rigoberto Ríos-Estepa, Juan F. Alzate |
| المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 1803, p 1803 (2022) International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1803 |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | human, splenic macrophages, Mycobacterium tuberculosis, clinical strains, dual RNA-seq, metabolic reconstruction, QH301-705.5, Organic Chemistry, General Medicine, Catalysis, Computer Science Applications, Inorganic Chemistry, Chemistry, Physical and Theoretical Chemistry, Biology (General), Molecular Biology, QD1-999, Spectroscopy |
| الوصف: | Tuberculosis (TB) is caused by Mycobacterium tuberculosis (Mtb), leading to pulmonary and extrapulmonary TB, whereby Mtb is disseminated to many other organs and tissues. Dissemination occurs early during the disease, and bacteria can be found first in the lymph nodes adjacent to the lungs and then later in the extrapulmonary organs, including the spleen. The early global gene expression response of human tissue macrophages and intracellular clinical isolates of Mtb has been poorly studied. Using dual RNA-seq, we have explored the mRNA profiles of two closely related clinical strains of the Latin American and Mediterranean (LAM) family of Mtb in infected human splenic macrophages (hSMs). This work shows that these pathogens mediate a distinct host response despite their genetic similarity. Using a genome-scale host–pathogen metabolic reconstruction to analyze the data further, we highlight that the infecting Mtb strain also determines the metabolic response of both the host and pathogen. Thus, macrophage ontogeny and the genetic-derived program of Mtb direct the host–pathogen interaction. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77b21d0c938de63320be130cc3279b7a https://www.mdpi.com/1422-0067/23/3/1803 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....77b21d0c938de63320be130cc3279b7a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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