Thermotolerance responses in ripening berries of vitis vinifera l. cv muscat hamburg
| العنوان: | Thermotolerance responses in ripening berries of vitis vinifera l. cv muscat hamburg |
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| المؤلفون: | Pablo Carbonell-Bejerano, Carolina Royo, M. Carmen Antolín, Rafael Torres-Pérez, Gema Bravo, Eva Santa María, Jone Aguirreolea, José M. Martínez-Zapater, Diego Lijavetzky, Manuel Sánchez-Díaz |
| المساهمون: | Fundación Genoma España, Genome Canada, Ministerio de Ciencia e Innovación (España), Universidad Nacional de Cuyo |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| بيانات النشر: | Oxford University Press, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Otras Biotecnología Agropecuaria, Light, Physiology, Biotecnología Agropecuaria, Malates, Plant Science, Berry, Biology, Anthocyanins, Heat Shock Transcription Factors, MICROARRAY, Gene Expression Regulation, Plant, Botany, Vitis, Amino Acids, Vitis vinifera, Transcriptomics, TEMPERATURE, Heat-Shock Proteins, Oligonucleotide Array Sequence Analysis, Plant Proteins, Temperatures, Reverse Transcriptase Polymerase Chain Reaction, Temperature, purl.org/becyt/ford/4.4 [https], Gene Expression Regulation, Developmental, food and beverages, Ripening, Cell Biology, General Medicine, Berry ripening, Adaptation, Physiological, DNA-Binding Proteins, ABA, CIENCIAS AGRÍCOLAS, Fruit, Christian ministry, ANTHOCYANIN, Transcriptome, BERRY, purl.org/becyt/ford/4 [https], Metabolic Networks and Pathways, Abscisic Acid, Transcription Factors |
| الوصف: | Berry organoleptic properties are highly influenced by ripening environmental conditions. In this study, we used grapevine fruiting cuttings to follow berry ripening under different controlled conditions of temperature and irradiation intensity. Berries ripened at higher temperatures showed reduced anthocyanin accumulation and hastened ripening, leading to a characteristic drop in malic acid and total acidity. The GrapeGen GeneChip® combined with a newly developed GrapeGen 12Xv1 MapMan version were utilized for the functional analysis of berry transcriptomic differences after 2 week treatments from veraison onset. These analyses revealed the establishment of a thermotolerance response in berries under high temperatures marked by the induction of heat shock protein (HSP) chaperones and the repression of transmembrane transporter-encoding transcripts. The thermotolerance response was coincident with up-regulation of ERF subfamily transcription factors and increased ABA levels, suggesting their participation in the maintenance of the acclimation response. Lower expression of amino acid transporter-encoding transcripts at high temperature correlated with balanced amino acid content, suggesting a transcriptional compensation of temperature effects on protein and membrane stability to allow for completion of berry ripening. In contrast, the lower accumulation of anthocyanins and higher malate metabolization measured under high temperature might partly result from imbalance in the expression and function of their specific transmembrane transporters and expression changes in genes involved in their metabolic pathways. These results open up new views to improve our understanding of berry ripening under high temperatures. © 2013 The Author. This study was supported by Genoma España [within a collaborative agreement with Genome Canada (Grapegen Project)]; the Spanish Ministry of Science and Innovation [grant Nos. BIO2008-03892, BIO2011-26229]; a bilateral collaborative grant with the University of Cuyo (Mendoza, Argentina) [grant No. AR2009-0021]. |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77740814adba6559589c148f5a503cf6 http://hdl.handle.net/10261/144138 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....77740814adba6559589c148f5a503cf6 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |