Post-Ischemic Treatment of Recombinant Human Secretory Leukocyte Protease Inhibitor (rhSLPI) Reduced Myocardial Ischemia/Reperfusion Injury
| العنوان: | Post-Ischemic Treatment of Recombinant Human Secretory Leukocyte Protease Inhibitor (rhSLPI) Reduced Myocardial Ischemia/Reperfusion Injury |
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| المؤلفون: | Podsawee Mongkolpathumrat, Anusak Kijtawornrat, Sarawut Kumphune, Aussara Panya, Eakkapote Prompunt, Nipon Chattipakorn, S. Barrere-Lemaire |
| المساهمون: | Naresuan University, Chulalongkorn University [Bangkok], University of Phayao, Chiang Mai University (CMU), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Guerineau, Nathalie C. |
| المصدر: | Biomedicines, Vol 9, Iss 422, p 422 (2021) Biomedicines Biomedicines, MDPI, 2021, 9 (4), pp.422. ⟨10.3390/biomedicines9040422⟩ Volume 9 Issue 4 Biomedicines, 2021, 9 (4), pp.422. ⟨10.3390/biomedicines9040422⟩ |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, [SDV]Life Sciences [q-bio], Medicine (miscellaneous), Inflammation, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, 030204 cardiovascular system & hematology, Pharmacology, ischemia/reperfusion injury, Article, General Biochemistry, Genetics and Molecular Biology, post-ischemic treatment, Proinflammatory cytokine, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Lactate dehydrogenase, medicine, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Protein kinase B, lcsh:QH301-705.5, secretory leukocyte protease inhibitor (SLPI), Cardioprotection, business.industry, Proteolytic enzymes, medicine.disease, ischemic heart disease, 3. Good health, [SDV] Life Sciences [q-bio], 030104 developmental biology, chemistry, lcsh:Biology (General), cardioprotection, medicine.symptom, business, Reperfusion injury, SLPI |
| الوصف: | International audience; Myocardial ischemia/reperfusion (I/R) injury is a major cause of mortality and morbidity worldwide. Among factors contributing to I/R injury, proteolytic enzymes could also cause cellular injury, expand the injured area and induce inflammation, which then lead to cardiac dysfunction. Therefore, protease inhibition seems to provide therapeutic benefits. Previous studies showed the cardioprotective effect of secretory leukocyte protease inhibitor (SLPI) against myocardial I/R injury. However, the effect of a post-ischemic treatment with SLPI in an in vivo I/R model has never been investigated. In the present study, recombinant human (rh) SLPI (rhSLPI) was systemically injected during coronary artery occlusion or at the onset of reperfusion. The results show that post-ischemic treatment with rhSLPI could significantly reduce infarct size, Lactate Dehydrogenase (LDH) and Creatine kinase-MB (CK-MB) activity, inflammatory cytokines and protein carbonyl levels, as well as improving cardiac function. The cardioprotective effect of rhSLPI is associated with the attenuation of p38 MAPK phosphorylation, Bax, caspase-3 and -8 protein levels and enhancement of pro-survival kinase Akt and ERK1/2 phosphorylation. In summary, this is the first report showing the cardioprotective effects against myocardial I/R injury of post-ischemic treatments with rhSLPI in vivo. Thus, these results suggest that SLPI could be used as a novel therapeutic strategy to reduce myocardial I/R injury. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2227-9059 |
| DOI: | 10.3390/biomedicines9040422⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77661840d95575b5ebcb9bec80e20576 https://www.mdpi.com/2227-9059/9/4/422 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....77661840d95575b5ebcb9bec80e20576 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22279059 |
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| DOI: | 10.3390/biomedicines9040422⟩ |