Presynaptically localized cyclic GMP-dependent protein kinase 1 is a key determinant of spinal synaptic potentiation and pain hypersensitivity
| العنوان: | Presynaptically localized cyclic GMP-dependent protein kinase 1 is a key determinant of spinal synaptic potentiation and pain hypersensitivity |
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| المؤلفون: | Erika Polgár, Andrew J. Todd, Vijayan Gangadharan, Rohini Kuner, Franz Hofmann, Susanne Feil, Nitin Agarwal, Robert Feil, Thomas Kuner, Anke Tappe-Theodor, Gary R. Lewin, Ceng Luo, Rou-Gang Xie, Irmgard Tegeder, Da-Lu Liu, Martina Kurejova, Jens Schlossmann, San-Jue Hu, Kiran Kumar Bali |
| المساهمون: | MDC Library |
| المصدر: | PLoS Biology, Vol 10, Iss 3, p e1001283 (2012) PLoS Biology 10 (3): e1001283. PLoS Biology |
| بيانات النشر: | Public Library of Science (PLoS), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Patch-Clamp Techniques, Long-Term Potentiation, Synaptic Transmission, Substrate Specificity, Mice, chemistry.chemical_compound, Nerve Fibers, Ganglia, Spinal, Inositol 1,4,5-Trisphosphate Receptors, Phosphorylation, Biology (General), Neurotransmitter, Cyclic GMP-Dependent Protein Kinase Type I, Mice, Knockout, Behavior, Animal, Animal Behavior, General Neuroscience, Microfilament Proteins, Nociceptors, Long-term potentiation, Anatomy, Nociception, medicine.anatomical_structure, Hyperalgesia, Aminoquinolines, Nociceptor, cardiovascular system, NMDA receptor, medicine.symptom, Function and Dysfunction of the Nervous System, General Agricultural and Biological Sciences, Research Article, Signal Transduction, Inbred C57BL Mice, Spinal Ganglia, QH301-705.5, Pain, 570 Life Sciences, Biology, Neurotransmission, General Biochemistry, Genetics and Molecular Biology, 610 Medical Sciences, Medicine, ddc:570, Cyclic GMP-Dependent Protein Kinases, medicine, Animals, ddc:610, General Immunology and Microbiology, Phosphoproteins, Spinal cord, Knockout Mice, Mice, Inbred C57BL, Enzyme Activation, chemistry, nervous system, Guanylate Cyclase, Cell Adhesion Molecules, Neuroscience, Gene Deletion |
| الوصف: | Electrophysiological and behavioral experiments in mice reveal that a cGMP-dependent kinase amplifies neurotransmitter release from peripheral pain sensors, potentiates spinal synapses, and leads to exaggerated pain. Synaptic long-term potentiation (LTP) at spinal neurons directly communicating pain-specific inputs from the periphery to the brain has been proposed to serve as a trigger for pain hypersensitivity in pathological states. Previous studies have functionally implicated the NMDA receptor-NO pathway and the downstream second messenger, cGMP, in these processes. Because cGMP can broadly influence diverse ion-channels, kinases, and phosphodiesterases, pre- as well as post-synaptically, the precise identity of cGMP targets mediating spinal LTP, their mechanisms of action, and their locus in the spinal circuitry are still unclear. Here, we found that Protein Kinase G1 (PKG-I) localized presynaptically in nociceptor terminals plays an essential role in the expression of spinal LTP. Using the Cre-lox P system, we generated nociceptor-specific knockout mice lacking PKG-I specifically in presynaptic terminals of nociceptors in the spinal cord, but not in post-synaptic neurons or elsewhere (SNS-PKG-I−/− mice). Patch clamp recordings showed that activity-induced LTP at identified synapses between nociceptors and spinal neurons projecting to the periaqueductal grey (PAG) was completely abolished in SNS-PKG-I−/− mice, although basal synaptic transmission was not affected. Analyses of synaptic failure rates and paired-pulse ratios indicated a role for presynaptic PKG-I in regulating the probability of neurotransmitter release. Inositol 1,4,5-triphosphate receptor 1 and myosin light chain kinase were recruited as key phosphorylation targets of presynaptic PKG-I in nociceptive neurons. Finally, behavioural analyses in vivo showed marked defects in SNS-PKG-I−/− mice in several models of activity-induced nociceptive hypersensitivity, and pharmacological studies identified a clear contribution of PKG-I expressed in spinal terminals of nociceptors. Our results thus indicate that presynaptic mechanisms involving an increase in release probability from nociceptors are operational in the expression of synaptic LTP on spinal-PAG projection neurons and that PKG-I localized in presynaptic nociceptor terminals plays an essential role in this process to regulate pain sensitivity. Author Summary Pain is an important physiological function that protects our body from harm. Pain-sensing neurons, called nociceptors, transduce harmful stimuli into electrical signals and transmit this information to the brain via the spinal cord. When nociceptors are persistently activated, such as after injury, the connections they make with neurons in the spinal cord are altered in a process called synaptic long-term potentiation (LTP). In this study, we examine the molecular and cellular mechanisms of LTP at synapses from nociceptors onto spinal neurons. We use multiple experimental approaches in mice, from genetic to behavioural, to show that this form of LTP involves presynaptic events that unfold in nociceptors when they are repetitively activated. In particular, an enzyme activated by the second messenger cGMP, referred to as Protein Kinase G-I, phosphorylates presynaptic proteins and increases the release of neurotransmitters from nociceptor endings in the spinal cord. When we genetically silence Protein Kinase G-I or block its activation in nociceptors, inflammatory pain is markedly reduced at the behavioural level. These results clarify basic mechanisms of pathological pain and pave the way for new therapeutic approaches. |
| وصف الملف: | application/pdf; application/octet-stream |
| اللغة: | English |
| تدمد: | 1545-7885 1544-9173 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76cc9782b0b3e1d7688492c7ec43a6a7 http://europepmc.org/articles/PMC3302842?pdf=render |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....76cc9782b0b3e1d7688492c7ec43a6a7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15457885 15449173 |
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