Human airway mucus alters susceptibility of Pseudomonas aeruginosa biofilms to tobramycin, but not colistin
| العنوان: | Human airway mucus alters susceptibility of Pseudomonas aeruginosa biofilms to tobramycin, but not colistin |
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| المؤلفون: | Marius Hittinger, Xabier Murgia, Katherina Sewald, Claus-Michael Lehr, Konrad Schwarzkopf, Laura Müller, Susanne Häussler, Lorenz Siebenbürger, Armin Braun, Carsten Börger, Sabine Wronski |
| المساهمون: | Publica, HIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany. |
| المصدر: | The Journal of antimicrobial chemotherapy England |
| بيانات النشر: | Oxford University Press (OUP), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), airway device, medicine.drug_class, 030106 microbiology, Antibiotics, Context (language use), Microbial Sensitivity Tests, medicine.disease_cause, Cystic fibrosis, Microbiology, 03 medical and health sciences, fluids and secretions, antibiotic, Tobramycin, medicine, Humans, Pharmacology (medical), Pharmacology, Colistin, Pseudomonas aeruginosa, Chemistry, Biofilm, diffusion, biochemical phenomena, metabolism, and nutrition, respiratory system, medicine.disease, Mucus, Anti-Bacterial Agents, Trachea, 030104 developmental biology, Infectious Diseases, Biofilms, medicine.drug |
| الوصف: | Objectives In the context of cystic fibrosis, Pseudomonas aeruginosa biofilms often develop in the vicinity of airway mucus, which acts as a protective physical barrier to inhaled matter. However, mucus can also adsorb small drug molecules administered as aerosols, including antibiotics, thereby reducing their bioavailability. The efficacy of antibiotics is typically assessed by determining the MIC using in vitro assays. This widespread technique, however, does not consider either bacterial biofilm formation or the influence of mucus, both of which may act as diffusion barriers, potentially limiting antibiotic efficacy. Methods We grew P. aeruginosa biofilms in the presence or absence of human tracheal mucus and tested their susceptibility to tobramycin and colistin. Results A significant reduction of tobramycin efficacy was observed when P. aeruginosa biofilms were grown in the presence of mucus compared with those grown in the absence of mucus. Diffusion of tobramycin through mucus was reduced; however, this reduction was more pronounced in biofilm/mucus mixtures, suggesting that biofilms in the presence of mucus respond differently to antibiotic treatment. In contrast, the influence of mucus on colistin efficacy was almost negligible and no differences in mucus permeability were observed. Conclusions These findings underline the important role of mucus in the efficacy of anti-infective drugs. |
| تدمد: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dky241 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7523ebcfe25639de84e60db0166d118b https://doi.org/10.1093/jac/dky241 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7523ebcfe25639de84e60db0166d118b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602091 03057453 |
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| DOI: | 10.1093/jac/dky241 |