Distinct properties and functions of CTCF revealed by a rapidly inducible degron system
| العنوان: | Distinct properties and functions of CTCF revealed by a rapidly inducible degron system |
|---|---|
| المؤلفون: | Yemin Lan, Haoyue Zhang, Yu Zhang, Marit W. Vermunt, Belinda Giardine, Jacob M Tome, Guanjue Xiang, Jing Luan, Pablo Aurelio Gómez-García, Cheryl A. Keller, Zhe Zhang, Gerd A. Blobel, John T. Lis, Anran Huang, Melike Lakadamyali, Ross C. Hardison |
| المصدر: | Cell reports Cell Reports, Vol 34, Iss 8, Pp 108783-(2021) |
| بيانات النشر: | Cell Press, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, CCCTC-Binding Factor, Erythroblasts, Transcription, Genetic, transcription elongation stalling, cohesin, Biology, Molecular Dynamics Simulation, General Biochemistry, Genetics and Molecular Biology, Article, Cell Line, Cromatina, 03 medical and health sciences, Mice, 0302 clinical medicine, chromatin architecture, Transcriptional regulation, degradation dynamics, Animals, Gene, lcsh:QH301-705.5, residence time, Zinc finger transcription factor, Gene Editing, Binding Sites, Cohesin, CTCF, Chromatin Assembly and Disassembly, Chromatin, Single Molecule Imaging, Cell biology, Kinetics, Genòmica, 030104 developmental biology, lcsh:Biology (General), Proteolysis, Chromatin Immunoprecipitation Sequencing, Chromatin Loop, RNA Polymerase II, Degron, CRISPR-Cas Systems, 030217 neurology & neurosurgery, Protein Binding |
| الوصف: | SUMMARY CCCTC-binding factor (CTCF) is a conserved zinc finger transcription factor implicated in a wide range of functions, including genome organization, transcription activation, and elongation. To explore the basis for CTCF functional diversity, we coupled an auxin-induced degron system with precision nuclear run-on. Unexpectedly, oriented CTCF motifs in gene bodies are associated with transcriptional stalling in a manner independent of bound CTCF. Moreover, CTCF at different binding sites (CBSs) displays highly variable resistance to degradation. Motif sequence does not significantly predict degradation behavior, but location at chromatin boundaries and chromatin loop anchors, as well as co-occupancy with cohesin, are associated with delayed degradation. Single-molecule tracking experiments link chromatin residence time to CTCF degradation kinetics, which has ramifications regarding architectural CTCF functions. Our study highlights the heterogeneity of CBSs, uncovers properties specific to architecturally important CBSs, and provides insights into the basic processes of genome organization and transcription regulation. Graphical Abstract In brief Using an inducible CTCF degradation approach, Luan et al. show that sensitivity to degradation of CTCF on chromatin is highly variable, reflecting its diverse roles in chromatin architecture and transcription. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74741760808e21d05f4d171f61abe8c0 http://hdl.handle.net/10230/52910 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....74741760808e21d05f4d171f61abe8c0 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |