The TES gene at 7q31.1 is methylated in tumours and encodes a novel growth-suppressing LIM domain protein
| العنوان: | The TES gene at 7q31.1 is methylated in tumours and encodes a novel growth-suppressing LIM domain protein |
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| المؤلفون: | Donald M. Black, Edward S. Tobias, E MacKenzie, Robert McFarlane, Adam Hurlstone |
| المصدر: | Oncogene. 20:2844-2853 |
| بيانات النشر: | Springer Science and Business Media LLC, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Male, Cancer Research, Tumor suppressor gene, Molecular Sequence Data, Loss of Heterozygosity, Breast Neoplasms, Locus (genetics), Biology, medicine.disease_cause, Frameshift mutation, Neoplasms, Tumor Cells, Cultured, Genetics, medicine, Humans, Genes, Tumor Suppressor, Frameshift Mutation, Molecular Biology, Tumor Stem Cell Assay, LIM domain, Homeodomain Proteins, Ovarian Neoplasms, Base Sequence, Tumor Suppressor Proteins, Chromosome Mapping, Prostatic Neoplasms, RNA-Binding Proteins, food and beverages, Sequence Analysis, DNA, DNA Methylation, LIM Domain Proteins, Molecular biology, Pancreatic Neoplasms, Cytoskeletal Proteins, Testin, Urinary Bladder Neoplasms, CpG site, DNA methylation, CpG Islands, Female, Carcinogenesis, Chromosomes, Human, Pair 7, HeLa Cells |
| الوصف: | Many studies suggest that a multi-tissue tumour suppressor gene is located at human chromosome 7q31.1. We have cloned and characterized a novel gene at this locus. The TES gene lies within the minimal region of overlap of several LOH studies and appears to possess the properties of a tumour suppressor. TES is widely expressed and is predicted to encode a protein of 421 amino acids, with three C-terminal LIM domains. Mutation analysis of the coding TES exons in 21 human tumour-derived cell lines revealed the presence of a frameshift mutation in one allele in the breast cancer cell line ZR-75. Methylation of the CpG island at the 5' end of TES appears to be a remarkably frequent finding, occurring in seven out of 10 ovarian carcinomas and in each of the 30 tumour-derived cell lines tested. Moreover, forced expression of TES in HeLa or OVCAR5 cells, resulted in a profound reduction in growth potential, as determined by the colony formation assay. We believe that TES is a tumour suppressor gene that is inactivated primarily by transcriptional silencing resulting from CpG island methylation. |
| تدمد: | 1476-5594 0950-9232 |
| DOI: | 10.1038/sj.onc.1204433 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7257274bb6765f192fa695421110ac50 https://doi.org/10.1038/sj.onc.1204433 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7257274bb6765f192fa695421110ac50 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765594 09509232 |
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| DOI: | 10.1038/sj.onc.1204433 |