Numerical abnormalities of chromosome 7 in human prostate cancer detected by fluorescencein situ hybridization (FISH) on paraffin-embedded tissue sections with centromere-specific dna probes
| العنوان: | Numerical abnormalities of chromosome 7 in human prostate cancer detected by fluorescencein situ hybridization (FISH) on paraffin-embedded tissue sections with centromere-specific dna probes |
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| المؤلفون: | Susanne Enders, Manfred Bauchinger, Jürgen Breul, Herbert Braselmann, Lars Lehmann, Heinz Höfler, Heinz-Ulrich G. Weier, Horst Zitzelsberger, Albrecht Schilling, Sandor Szücs |
| المصدر: | The Journal of Pathology. 172:325-335 |
| بيانات النشر: | Wiley, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Male, Pathology, medicine.medical_specialty, Centromere, Trisomy, Biology, Pathology and Forensic Medicine, Polyploidy, Polysomy 7, Parenchyma, medicine, Carcinoma, Humans, Interphase, In Situ Hybridization, Fluorescence, Chromosome Aberrations, Chromosome 7 (human), Chromosomes, Human, Pair 12, Paraffin Embedding, medicine.diagnostic_test, Hybridization probe, Cytogenetics, Prostatic Neoplasms, Cancer, medicine.disease, DNA Probes, Chromosomes, Human, Pair 7, Chromosomes, Human, Pair 8, Fluorescence in situ hybridization |
| الوصف: | Fluorescence in situ hybridization (FISH) using chromosome-specific α-satellite DNA probes for chromosomes 7, 8, and 12 was performed on paraffin-embedded tissue sections and touch imprint preparations of 53 cases of human prostate cancer. Subsequent haematoxylin and eosin (H & E) staining of the hybridized tissue sections allowed unambiguous assignment of hybridization signals either to tumour or to non-tumorous parenchyma. Fifty-three cases of human prostate cancer were evaluated for numerical aberrations of chromosome 7. Scoring 200 cells of tumour and non-tumorous parenchyma in each case revealed abnormalities exclusively in tumour parenchyma in 41 cases (77 per cent). Ten of 41 cases (24 per cent) showed trisomy 7, and 15 cases (37 per cent) monosomy 7 or trisomy 7 in combination with monosomy 7, respectively. Sixteen cases (39 per cent) exhibited polysemy 7 in cells of the tumour parenchyma. In the tumour tissue in one case, different polyploid clones (triploid, tetraploid) and polysomy 7 could be identified by double hybridization with chromosome-specific DNA probes for chromosome 7, plus 8 or 12. The indicated numerical aberrations of chromosome 7 were correlated with 78 per cent of advanced pathological stages or poorly differentiated tumours (pT3/4 or G3) of prostate carcinomas. A statistical analysis of the data revealed significant relationships of particular numerical abnormalities of chromosome 7 to different pathological categories (pT, G, pN) of tumour classification. For the T-classification, the frequency of cells carrying polysomy 7 and polysomy 7/+7 increases significantly from pT1 to pT3/4 (P = 0·022). A significant increase from G1 to G3 also became apparent for the total frequencies of numerical abnormal cells (P = 0·05). |
| تدمد: | 1096-9896 0022-3417 |
| DOI: | 10.1002/path.1711720407 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f9c035eb02f716f65ffc000d1ed3d49 https://doi.org/10.1002/path.1711720407 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....6f9c035eb02f716f65ffc000d1ed3d49 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10969896 00223417 |
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| DOI: | 10.1002/path.1711720407 |