The importance of the urea cycle and its relationships to polyamine metabolism during ammonium stress in Medicago truncatula
| العنوان: | The importance of the urea cycle and its relationships to polyamine metabolism during ammonium stress in Medicago truncatula |
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| المؤلفون: | Marina Urra, Javier Buezo, Beatriz Royo, Alfonso Cornejo, Pedro López-Gómez, Daniel Cerdán, Raquel Esteban, Víctor Martínez-Merino, Yolanda Gogorcena, Paraskevi Tavladoraki, Jose Fernando Moran |
| المساهمون: | Ministerio de Ciencia e Innovación (España), Ministerio de Economía y Competitividad (España), Eusko Jaurlaritza, Diputación Foral de Navarra, Ministero dell'Istruzione, dell'Università e della Ricerca, Università degli Studi Roma Tre, Agencia Estatal de Investigación (España), Gobierno de Aragón, Universidad Pública de Navarra, Gogorcena Aoiz, Yolanda, Universidad Pública de Navarra. Departamento de Ciencias, Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa. INAMAT2 - Institute for Advanced Materials and Mathematics, Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa. IMAB - Institute for Multidisciplinary Research in Applied Biology, Nafarroako Unibertsitate Publikoa. Zientziak Saila, Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, Gobierno de Navarra / Nafarroako Gobernuaren, Gogorcena Aoiz, Yolanda [0000-0003-1081-430X] |
| المصدر: | Addi. Archivo Digital para la Docencia y la Investigación Universidad de Cantabria (UC) Academica-e. Repositorio Institucional de la Universidad Pública de Navarra instname Digital.CSIC. Repositorio Institucional del CSIC |
| بيانات النشر: | Oxford University Press (OUP), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Ornithine, amine oxidase, nitrogen nutrition, Spermidine, Physiology, ornithine-decarbxylase, toxicity, Plant Science, copper amine oxidase, ammonium stress, arabidopsis, synthetase, polyamine, glycine-max, nitrate, Ammonium Compounds, Medicago truncatula, Polyamines, urea cycle, Urea, putrescine, acid, Amine Oxidase (Copper-Containing), plant development, biosynthesis |
| الوصف: | 15 Pags.- 11 Figs. © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. The ornithine–urea cycle (urea cycle) makes a significant contribution to the metabolic responses of lower photosynthetic eukaryotes to episodes of high nitrogen availability. In this study, we compared the role of the plant urea cycle and its relationships to polyamine metabolism in ammonium-fed and nitrate-fed Medicago truncatula plants. High ammonium resulted in the accumulation of ammonium and pathway intermediates, particularly glutamine, arginine, ornithine, and putrescine. Arginine decarboxylase activity was decreased in roots, suggesting that the ornithine decarboxylase-dependent production of putrescine was important in situations of ammonium stress. The activity of copper amine oxidase, which releases ammonium from putrescine, was significantly decreased in both shoots and roots. In addition, physiological concentrations of ammonium inhibited copper amine oxidase activity in in vitro assays, supporting the conclusion that high ammonium accumulation favors putrescine synthesis. Moreover, early supplementation of plants with putrescine avoided ammonium toxicity. The levels of transcripts encoding urea-cycle-related proteins were increased and transcripts involved in polyamine catabolism were decreased under high ammonium concentrations. We conclude that the urea cycle and associated polyamine metabolism function as important protective mechanisms limiting ammonium toxicity in M. truncatula. These findings demonstrate the relevance of the urea cycle to polyamine metabolism in higher plants. This work was supported by the grants from the Spanish Government AGL2014-52396-P (MICINN) and AGL2017-86293-P (MINECO/FEDER) to JFM, and the Basque Government, Spain, IT-1018-16 (UPV/EHU-GV) to RE. MU is a recipient of a pre-doctoral fellowship from the Government of Navarre, Spain. JB and PLG have received pre-doctoral fellowships from the Public University of Navarre, Spain. PT has received funding from the Italian Ministry of Education, University and Research (Grant to Department of Science, University ‘Roma Tre’-‘Dipartimenti di Eccellenza’, ARTICOLO 1, COMMI 314–337. LEGGE 423 232/2016; PRIN 2017—CUP F84I19000730005). Partial support was obtained from the Spanish State Research Agency AGL2017-83358-R (AEI/FEDER) and the Government of Aragón, Spain, Group A09-20R to YG. Open Access funding was provided by the Public University of Navarra. |
| وصف الملف: | application/pdf |
| تدمد: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/erac235 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ec26e66ae87eedae36f86676a3c5a36 https://doi.org/10.1093/jxb/erac235 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6ec26e66ae87eedae36f86676a3c5a36 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602431 00220957 |
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| DOI: | 10.1093/jxb/erac235 |