Morphological Changes and Expression of Cytokine After Local Endometrial Injury in a Mouse Model
| العنوان: | Morphological Changes and Expression of Cytokine After Local Endometrial Injury in a Mouse Model |
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| المؤلفون: | Yong-Hong Zhang, Zhao-Zhao Liu, Xiao-Hui Zhang, Lian Hu, Ai-Hua Liao, Mao-Xing Tang |
| المصدر: | Reproductive Sciences. 22:1377-1386 |
| بيانات النشر: | Springer Science and Business Media LLC, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Time Factors, medicine.medical_treatment, Uterus, Gestational Age, Wounds, Penetrating, Oncostatin M, Biology, Leukemia Inhibitory Factor, Andrology, Endometrium, Mice, Downregulation and upregulation, Pregnancy, medicine, Animals, Embryo Implantation, RNA, Messenger, Estrous cycle, Messenger RNA, Gene Expression Regulation, Developmental, Obstetrics and Gynecology, Embryo, Up-Regulation, Disease Models, Animal, Cytokine, medicine.anatomical_structure, Immunology, biology.protein, Cytokines, Female, Leukemia inhibitory factor, Signal Transduction |
| الوصف: | To establish a mouse model for endometrial injury and determine the underlying mechanism regarding its favorable effect on embryo implantation. Female Kunming mice were randomly allocated into 4 groups: group I, normal control; group II, injury procedure control; and group III and group IV, the mice being scratched with a blunt syringe on the right uterine horn or both, respectively. All the mice were mated with the males during the next estrus phase. The number of implanted embryos on each side of uterus was calculated on day 8 of pregnancy. The endometrial samples were taken on day 4 of pregnancy, and the local morphological changes and cytokine expressions were examined. Compared to group II, our results showed that in group IV (1) there were significantly higher numbers of implanted embryos, (2) the endometrial glands and vasculatures in stroma were obviously increased and the pinopodes were abundant and well developed, and (3) the local levels of cytokines leukemia inhibitory factor (LIF) and oncostatin M (OSM) messenger RNA and protein expression were significantly increased. Local mechanical injury on mouse uteri enhanced endometrial receptivity and improved embryo implantation, which were correlated with the characteristic changes in endometrial morphology and the upregulation of LIF and OSM gene and protein expression. |
| تدمد: | 1933-7205 1933-7191 |
| DOI: | 10.1177/1933719115580999 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e4ea853f41f9ad1a6f20bc9999cb985 https://doi.org/10.1177/1933719115580999 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....6e4ea853f41f9ad1a6f20bc9999cb985 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19337205 19337191 |
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| DOI: | 10.1177/1933719115580999 |